A9792

抗ニワトリIgY (IgG) (全分子)−ペルオキシダーゼ ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: Rabbit Anti-Chicken IgY (IgG) (whole molecule)−HRP
• MDL番号: MFCD00162225
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 結合体: peroxidase conjugate
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 交差性: chicken
• テクニック: direct ELISA: 1:30,000; dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate.; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

特異性

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgY.

免疫原

purified chicken IgY (IgG)

アプリケーション

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used in immunohistology.

Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution.

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for ELISA. The antibody can also be used for dot blot (1:160,000) and IHC (1:500) assays.

生物化学的／生理学的作用

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

調製ノート

Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978)による2段階グルタルアルデヒド法により作製しています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H317,H334
• 注意書き: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• 危険有害性の分類: Resp. Sens. 1 - Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable