おすすめの製品
由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
hABH2-7, monoclonal
フォーム
buffered aqueous solution
分子量
antigen ~30 kDa
化学種の反応性
human
テクニック
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of 293T cells
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ALKBH2(121642)
詳細
Anti-hABH2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH2-7 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human hABH2, conjugated to KLH. Human alkB homolog 1, histone H2A dioxygenase (hABH2) shares homology with the AlkB protein in bacteria. hABH2 localizes to the nucleoplasm and occasionally to the nucleoli.
アプリケーション
Mouse Monoclonal Anti-hABH2 antibody has been used for western blot assays. The antibody can also be used for IHC, ELISA, microarray, and immunoprecipitation studies.
生物化学的／生理学的作用
Removal of the methyl groups of 1-methyladenine (1-meA) and 3-methylcystosine (3-meC) to their unmodified forms is done by human alkB homolog 1, histone H2A dioxygenase (hABH2) and hABH3. hABH2 acts on double stranded DNA.
hABH2 is a Fe (II) dependent dioxygenase involved in the repair of alkylation-induced damage in double stranded DNA. Alterations in hABH2 have been linked to pediatric brain tumor and gastric cancer.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A8228-VAR:
A8228-BULK:
A8228-200UL:
Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase
Koivisto P, et al.
The Journal of Biological Chemistry, 278(45), 44348-44354 (2003)
Reversal of DNA alkylation damage by two human dioxygenases
Duncan T, et al.
Proceedings of the National Academy of Sciences of the USA, 99(26), 16660-16665 (2002)
Wei Gao et al.
Journal of gastroenterology and hepatology, 26(3), 577-584 (2010-12-16)
Methyl or 1, N(6) -ethenoadenine base lesions are frequent and highly-mutagenic or -carcinogenic events in mammalian DNA. Human AlkB homologue-2 (hABH2), a homologue of the Escherichia coli AlkB protein, has been found to be the principal dioxygenase for the repair
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
Aas PA, et al.
Nature, 421(6925), 859-859 (2003)
Tor-Christian Aase Johannessen et al.
Neuro-oncology, 15(3), 269-278 (2012-12-22)
Glioblastoma multiforme (GBM; World Health Organization astrocytoma grade IV) is the most frequent and most malignant primary brain tumor in adults. Despite multimodal therapy, all such tumors practically recur during the course of therapy, causing a median survival of only
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