A7005

β-アミラーゼ from sweet potato

Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

• 別名: 1,4-α-D-グルカン マルトヒドロラーゼ
• CAS番号: 9000-91-3
• Enzyme Commission number: 3.2.1.2 (BRENDA, IUBMB)
• EC Number: 232-566-1
• MDL番号: MFCD00081391
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: sweet potato
• 品質水準: 200
• タイプ: Type I-B
• 形状: ammonium sulfate suspension
• 比活性: ≥750 units/mg protein (E1%/280)
• 環境により配慮した代替製品スコア: old score: 57; new score: 1; Find out more about DOZN™ Scoring
• 環境により配慮した代替製品の特徴: Waste Prevention; Safer Solvents and Auxiliaries; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 濃度: ≥7.0 mg/mL
• 環境により配慮した代替製品カテゴリ: , Re-engineered
• 保管温度: 2-8°C

詳細

詳細

β-Amylases belongs to the class of amylolytic enzymes.

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Enhanced Energy Efficiency”. Click here to view its DOZN scorecard.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

アプリケーション

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.

β-Amylase from sweet potato has been used:

• in the hydrolysis of starch and starch-pectin combinations
• for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay
• for selenium speciation in samples

生物化学的／生理学的作用

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.

β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.

単位の定義

1ユニットは、pH 4.8、20°C、3分間に、1.0 mgのマルトースをデンプンから遊離させる酵素量です。

物理的形状

2.3 Mの(NH₄)₂SO₄溶液で調製した結晶性懸濁液

その他情報

より詳細な情報につきましては、www.sigma-aldrich.com/enzymeexplorerのenzymes for complex carbohydrate analysisをご覧ください。

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P342 + P311
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3