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由来生物
rabbit
品質水準
結合体
alkaline phosphatase conjugate
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
secondary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
化学種の反応性
mouse
テクニック
direct ELISA: 1:15,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue
western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL)
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.
免疫原
purified mouse IgG
アプリケーション
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :
- direct enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunohistochemistry
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.
生物化学的／生理学的作用
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization.
物理的形状
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A2418-BULK:
A2418-1ML:
A2418-VAR:
Guangyi Qin et al.
Oncology letters, 15(6), 8756-8760 (2018-05-29)
The present study aimed to investigate the effect of allyl isothiocyanate (AITC) on the viability and apoptosis of the human cervical cancer HeLa cell line in vitro, and to explore the potential underlying mechanisms of this. HeLa cells were treated
S Mohan et al.
Parasitology, 123(Pt 3), 271-276 (2001-10-02)
Pasteuria penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken
Santos C, et al.
Scientia Agricola, 65(5), 453-458 (2008)
C M Niemeyer et al.
Nucleic acids research, 22(25), 5530-5539 (1994-12-25)
Modified biomolecules were used for the non-covalent assembly of novel bioconjugates. Hybrid molecules were synthesized from short single-stranded DNA and streptavidin by chemical methods using a heterobispecific crosslinker. The covalent attachment of an oligonucleotide moiety to streptavidin provides a specific
Structure and function of human and murine receptors for IgG
Unkeless J C, et al.
Annual Review of Immunology, 6(1), 251-281 (1988)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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