A4312

抗マウスIgG (全分子)−アルカリフォスファターゼ ウサギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Rabbit Anti-Mouse IgG (whole molecule)−AP
• MDL番号: MFCD00162640
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous glycerol solution
• 交差性: mouse
• テクニック: direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000 using using β-Actin in total cell extract of HeLa cells (5-10 μg/well)
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid, capable of fighting bacterial and viral infection.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

免疫原

Purified mouse IgG.

アプリケーション

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in immunocytochemical localization of Aβ peptides and western blot analysis.

Anti-mouse IgG (whole molecule)-alkaline phosphatase antibody can be used in immunocytochemistry.

Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

生物化学的／生理学的作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

物理的形状

0.05 M Trisバッファ-溶液 (pH 8.0, 1mM MgCl₂, 10mM グリシン, 1%BSA, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable