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由来生物
human cervix
品質水準
形状
liquid
成長モード
Adherent
核型
2n = 46
形態
Epithelial
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Human cervix carcinoma
細胞株の説明
These cells were originally Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival ′D′ cells. Susceptible to swine influenza viruses, supports production of plaque formation by a variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Epithelial with occasional giant cells. Ethnicity: Black.
アプリケーション
Plaque assays, cloning, genetic and virus studies: swine influenza viruses
培地
Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
継代と培養方法
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using EDTA or 0.25% trypsin; 5% CO2; 37°C.
その他情報
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
In vitro, 14(5), 469-475 (1978-05-01)
Seed stocks of human cell lines deposited at the American Type Culture Collection (ATCC) have been examined for cross-contamination with HeLa cells using Giemsabanded marker chromosomes. Sixteen additional cell lines investigated have been found to exhibit marker chromosomes typical of
Continuous subcultivation of epithelial-like cells from normal human tissues.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 87(2), 440-443 (1954-11-01)
Examination of ATCC stocks for HeLa marker chromosomes in human cell lines.
Nature, 259(5540), 211-213 (1976-01-22)
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