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product name
Chang Liver (HeLa derivative) cell line,
由来生物
human cervix
形状
liquid
成長モード
Adherent
核型
Modal no. 70
形態
Epithelial
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Human cervix carcinoma
細胞株の説明
The cell line Chang Liver was originally thought to be derived from normal liver. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
アプリケーション
Chang Liver (HeLa derivative) cell line has been used to study the construction and biological activity of human torque teno virus (TTV) genotype 6.
Virus, biochemical and replication studies
DNAプロファイル
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,14
D16S539: 9,10
D5S818: 12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
CSF1PO: 10
D13S317: 12,14
D16S539: 9,10
D5S818: 12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
培地
EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）
継代と培養方法
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
その他情報
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Kofi D Kwofie et al.
Antimicrobial agents and chemotherapy, 60(6), 3283-3290 (2016-03-10)
Trypanosoma brucei parasites are kinetoplastid protozoa that devastate the health and economic well-being of millions of people in Africa through the disease human African trypanosomiasis (HAT). New chemotherapy has been eagerly awaited due to severe side effects and the drug
Construction and biological activity of a full?length molecular clone of human Torque teno virus (TTV) genotype 6
Kakkola L, et al.
FEBS Journal, 274(18), 4719-4730 (2007)
Clonal variation and interaction of cells with viruses.
W H MURPHY et al.
National Cancer Institute monograph, 7, 249-271 (1962-04-01)
Continuous subcultivation of epithelial-like cells from normal human tissues.
R S M CHANG
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 87(2), 440-443 (1954-11-01)
Takuhiro Uto et al.
The American journal of Chinese medicine, 43(4), 757-767 (2015-06-30)
Alnus japonica Steud is a tree that grows in damp areas of mountain valleys and has been used as a traditional medicine in Asia. We investigated the antiproliferative activity of hirsutanone (Hir) and oregonin (Ore) in human cancer cell lines
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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