69890

MES 水和物

BioUltra, ≥99.5% (T)

• 別名: 2-(N-モルホリノ)エタンスルホン酸 水和物, 4-モルホリンエタンスルホン酸
• 実験式（ヒル表記法）: C₆H₁₃NO₄S · xH₂O
• CAS番号: 1266615-59-1
• 分子量: 195.24 (anhydrous basis)
• MDL番号: MFCD00149409
• UNSPSCコード: 12161700
• eCl@ss: 32129211
• PubChem Substance ID: 57652220
• NACRES: NA.25

特性

• 製品種目: BioUltra
• 品質水準: 200
• アッセイ: ≥99.5% (T)
• 形状: powder or crystals
• 不純物: insoluble matter, passes filter test
• 強熱残分(900 °C): ≤0.05%
• pH: 2.5-4.0 (25 °C, 0.5 M in H₂O)
• 有効pH範囲: 5.5-6.7
• pKa: 6.1
• 溶解性: H₂O: 0.5 M at 20 °C, clear, colorless
• 微量陰イオン: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• 微量陽イオン: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤20 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV吸収: λ: 260 nm A_max: 0.025; λ: 280 nm A_max: 0.020
• SMILES記法: O.OS(=O)(=O)CCN1CCOCC1
• InChI: 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
• InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N

詳細

詳細

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

調製ノート

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

その他情報

MES水和物製品を簡単に比較検討できるMES水和物規格表をご参照ください。

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

生物学用バッファ-であり、有効pH範囲は5.8~6.5。アミノ活性化ゲルクロマトグラフィ-による脳チュ-ブリン分離における移動相の成分。植物組織培養で用いられるバッファ-。

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves