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Merck

50185

Sigma-Aldrich

Anti-Mouse-IgG - Atto 647N antibody produced in goat

contains 50% glycerol as stabilizer

別名:

Atto 647N-Anti-Mouse-IgG antibody produced in goat

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

結合体

Atto 647N conjugate

品質水準

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

secondary antibodies

クローン

polyclonal

含みます

50% glycerol as stabilizer

化学種の反応性

mouse

濃度

≥0.8 mg/mL IgG

テクニック

immunofluorescence: 5 μg/mL

蛍光検出

λex 647 nm; λem 665 nm in PBS

適合性

in accordance for fluorescence

輸送温度

wet ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

関連するカテゴリー

詳細

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Atto 647N-goat anti-mouse IgG associates with mouse IgGs.

免疫原

mouse IgG

アプリケーション

Solution in 0.1 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.5
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

物理的形状

Solution in 0.1 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.5

アナリシスノート

Das eingesetzte Farbtstoff/IgG-Verhältnis beträgt ~4:1
frei von unkonjugiertem Farbstoff

法的情報

本製品は研究専用です。商業目的で使用する場合は、ライセンス取得について知的所有権(IP)保持者(ATTO-TEC GmbH、ドイツ)にお問い合わせください。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

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Beatriz Marcos-Ramiro et al.
The Journal of cell biology, 213(3), 385-402 (2016-05-04)
Endothelial barrier dysfunction underlies chronic inflammatory diseases. In searching for new proteins essential to the human endothelial inflammatory response, we have found that the endosomal GTPase RhoB is up-regulated in response to inflammatory cytokines and expressed in the endothelium of
Zoran V Popovic et al.
Scientific reports, 7(1), 311-311 (2017-03-24)
Tissue osmolarity varies among different organs and can be considerably increased under pathologic conditions. Hyperosmolarity has been associated with altered stimulatory properties of immune cells, especially macrophages and dendritic cells. We have recently reported that dendritic cells upon exposure to
H Singh et al.
Neuroscience, 317, 76-107 (2016-01-17)
Large conductance voltage and calcium-activated potassium (MaxiK) channels are activated by membrane depolarization and elevated cytosolic Ca(2+). In the brain, they localize to neurons and astrocytes, where they play roles such as resetting the membrane potential during an action potential
Audrey Durand et al.
Nature communications, 9(1), 5247-5247 (2018-12-12)
Traditional approaches for finding well-performing parameterizations of complex imaging systems, such as super-resolution microscopes rely on an extensive exploration phase over the illumination and acquisition settings, prior to the imaging task. This strategy suffers from several issues: it requires a
Jasmin Mertins et al.
eLife, 10 (2021-11-16)
SNARE proteins have been described as the effectors of fusion events in the secretory pathway more than two decades ago. The strong interactions between SNARE domains are clearly important in membrane fusion, but it is unclear whether they are involved

資料

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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