408R-1

S100A1 (EP184) Rabbit Monoclonal Primary Antibody

• UNSPSCコード: 12352203
• NACRES: NA.41
• クローン: EP184, monoclonal
• application: IHC (p)
• 化学種の反応性: human
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
• citations: 2

特性

• 由来生物: rabbit
• 品質水準: 100; 500
• 結合体: unconjugated
• 抗体製品の状態: culture supernatant
• 抗体製品タイプ: primary antibodies
• クローン: EP184, monoclonal
• 詳細: For In Vitro Diagnostic Use in Select Regions (See Chart)
• フォーム: buffered aqueous solution
• 化学種の反応性: human
• 包装: vial of 0.1 mL concentrate (408R-14); vial of 0.5 mL concentrate (408R-15); bottle of 1.0 mL predilute (408R-17); vial of 1.0 mL concentrate (408R-16); bottle of 7.0 mL predilute (408R-18)
• メーカー／製品名: Cell Marque^™
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
• アイソタイプ: IgG
• コントロール: kidney, renal oncocytoma
• 輸送温度: wet ice
• 保管温度: 2-8°C
• 視覚化: cytoplasmic, nuclear
• 遺伝子情報: human ... S100A1(6271)

詳細

詳細

S100A1 is a member of S100 protein family of calcium binding proteins.S100A1 is very useful in the identification of renal cell carcinoma.Published literature indicates that 37 of 40 renal oncocytomas (93%), including two with the tubulo-cystic growth pattern, reacted positively by anti-S100A1.Most of them showed both cytoplasmic and nuclear immunostaining with moderate-to-strong intensity. S100A1 immunostaining was also observed in 30 of 41 clear cell renal cell carcinomas (73%); 6 showed focal positivity, whereas 24 displayed an immunoreactivity ranging from moderate (12 cases) to diffuse (12 cases) expression. Anti-S100A1 detected 30 cases of 32 papillary renal cell carcinomas (94%), 6 showing focal, 12 moderate, and 12 diffuse immunoreactivity. This study also demonstrated that 48 of the 51 chromophobe renal cell carcinomas (94%) showed no labeling by anti-S100A1. In 3 cases of chromophobe renal cell carcinomas (6%) that were positive by anti-S100A1, 2 were classic variant showing scattered S100A1 immunoreactive cells and an eosinophilic variant with nucleocytoplasmic staining in about 40% of the neoplastic cells.Therefore, anti-S100A1 is very useful in the differentiation of renal oncocytoma from chromophobe renal cell carcinoma as well as distinguishing clear cell renal cell carcinoma from chromophobe renal cell carcinoma. S100A1 is detected in normal renal tissue and expressed in both the nuclei and the cytoplasm of the cells lining proximal tubules, loops of Henle, and collecting ducts. The glomerular components are negative. S100A1 is also expressed by skeletal muscle and myocardium; and specific cytoplasmic expression is found in follicular dendritic cells of lymph node.

品質

IVD

IVD

IVD

RUO

関連事項

S100A1 Positive Control Slides, Product No. 408S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

物理的形状

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

調製ノート

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

その他情報

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

法的情報

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable