408OA-05A

Human Fibroblast-Like Synoviocytes-Osteoarthritis: HFLS-OA, adult

• 別名: Human fibroblast culture
• UNSPSCコード: 41106514
• NACRES: NA.81

特性

• 由来生物: human synovial tissues (osteoarthritic)
• 品質水準: 100
• 包装: pkg of 500,000 cells
• メーカー／製品名: Cell Applications, Inc
• 成長モード: Adherent
• 核型: 2n = 46
• 形態: Fibroblast-like
• テクニック: cell culture | mammalian: suitable
• 関連疾患: arthritis; coagulation disorders
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

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HFLS-RA provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of rheumatoid arthritis.

HFLS-RA have been used in numerous research studies to:

• Study signaling pathways implicated in the development of joint inflammation and rheumatoid arthritis (Abe, 2006; Akasaka, 2005; Bai, 2012; Fan, 2013; Furuhashi, 2008; Hashizume, 2008; Matsuyama, 2010; Tsuji, 1999; Yamaguch, 2012a,b; Yano, 2001)
• Evaluate possible contribution of EBV to nonresolving rheumatoid arthritis inflammation through inducing IL-6 production by synoviocytes (Chiu, 2013)
• Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as celastrol (Venkatesha, 2011; Xu, 2013), triptolide (Kong, 2013; Lin, 2013), arsenic trioxide (Mei, 2011), Wen Luo Yin (Liu, 2013), bucillamine (Tsuji, 2007), therapeutic lyposomes (Harigai, 2007; Ichihara, 2011; Matsumoto, 2013; Negishi, 2011), anti-IL-6R and anti-CD319 antibodies (Suzuki, 2010; Woo, 2013), as well as inhibitors of NF-κB (Baima, 2010; Keith, 2005), methionine aminopeptidase-2 (Bernier, 2004), Tpl2 (Hall, 2007), aryl hydrocarbon receptor (Lahoti, 2013) and p38 MAP kinase (Wada, 2005); and additionally demonstrate the beneficial anti-inflammatory effects of low level light therapy (Yamaura, 2009)
• Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001)
• Develop adenovirus-based siRNA delivery system (Arts, 2003)

Additionally, together with normal HFLS, HFLS-RA were used to:

• Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
• Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
• Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)

In parallel with HFLS isolated from joints of patients with osteoarthritis HFLS-RA were used in a study demonstrating the involvement of capsid proteins of parvorvirus B19 in activating synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy (Lu, 2006).

Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).

細胞株の由来

Bone

アプリケーション

synoviocyte physiology in relation to development and treatment of rheumatoid arthritis, phenotype, proinflammatory mediator production, signaling pathways, interleukin production, evaluation of anti-inflammatory and antirheumatic compounds, erosive enzyme production (matrix metalloproteinases), cell proliferation and attraction

構成

Basal Medium containing 10% FBS & 10% DMSO

調製ノート

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 5 doublings

継代と培養方法

Please refer to the HFLS-OA Culture Protocol.

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable