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由来生物
human articular cartilage
品質水準
包装
pkg of 500,000 cells
メーカー/製品名
Cell Applications, Inc
成長モード
Adherent
核型
2n = 46
形態
chondrocyte
テクニック
cell culture | mammalian: suitable
関連疾患
arthritis
輸送温度
dry ice
保管温度
−196°C
詳細
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Human Chondrocytes, HC, are derived from normal human articular cartilage where they produce and maintain the extracellular matrix of cartilage, including type II collagen. Chondrocytes grown in monolayer culture on a solid surface tend to lose their phenotypic markers and became de-differentiated to a fibroblast-like phenotype. This de-differentiation stage can be reversed by culturing them in a semi-solid gel.
Normal Chondrocytes obtained from Cell Applications, Inc. have been adapted as an in vitro model system in multiple studies looking for cellular mechanisms, such as inflammation-related signaling cascades, abnormal proteinase production, chondrocyte apoptosis and differentiation, as well as novel potential treatments for arthritic disease.
Characterization: Positive for aggrecan after differentiation.
Normal Human Chondrocytes (HC) have been used to:
Normal Chondrocytes were also utilized in a functional cluster formation agarose assay that allows chondrocytes to maintain their differentiated state (Quintavalla, 2005) and to investigate integrin-mediated mechanotransduction pathways required for proper chondrocyte function (Whitney, 2012). Additionally, they have used extensively in material studies aimed to improve chondrocyte adhesion to medical implants (Gutwein, 2002; Ellison, 2003; Price, 2004; Savaiano, 2004; Burns, 2009) and scaffolds for cartilage regeneration (Jun, 2002; Kay, 2002; Miller, 2002a,b; Price, 2002, 2003; Rao, 2004; Park, 2005; Khang, 2008). Normal chondrocyte RNA was used as a gold standard control in research on cellular reprogramming into chondrocytes (Ishii, 2012).
Human Chondrocytes, HC, are derived from normal human articular cartilage where they produce and maintain the extracellular matrix of cartilage, including type II collagen. Chondrocytes grown in monolayer culture on a solid surface tend to lose their phenotypic markers and became de-differentiated to a fibroblast-like phenotype. This de-differentiation stage can be reversed by culturing them in a semi-solid gel.
Normal Chondrocytes obtained from Cell Applications, Inc. have been adapted as an in vitro model system in multiple studies looking for cellular mechanisms, such as inflammation-related signaling cascades, abnormal proteinase production, chondrocyte apoptosis and differentiation, as well as novel potential treatments for arthritic disease.
Characterization: Positive for aggrecan after differentiation.
Normal Human Chondrocytes (HC) have been used to:
- Develop a high throughput assay to screen for genes capable of inducing an OA-like phenotype in chondrocytes in order to identify key pathways implicated in the disease (Daouti, 2005)
- Elucidate the signaling cascade leading to induction of proinflammatory cytokines by chondrocytes in RA joints (Aida, 2006; Wang, 2011a,b) and effects of elevated IL-6 signaling on normal chondrocytes (Namba, 2007)
- Investigate mechanisms and identify inhibitors of increased production of proteinases by chondrocytes stimulated by interleukin-1 (Aida, 2005; Wada, 2006), retinoic acid (Hikichi, 2009), proinflammatory cytokines (Tanigawa, 2011a,b), nitric oxide (Wu, 2007; Yang, 2011) and shear stress (Wang, 2011b, 2012);
- Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001; Behera, 2004, 2005)
- Study the factors affecting chondrocyte apoptosis (Cherng, 2008; Malemud, 2012) and differentiation into osteoclasts (Watanabe, 2009a,b; Honda, 2011)
- Show that hyaluronate (HA) can prevent the aggravated cartilage degradation by blocking the matrix metalloproteinases production from cytokine-activated chondrocytes via MKP-1 induction through CD44 signaling (Hashizume, 2009, 2010)
- Demonstrate cytotoxic effects of anti-human Fas/APO-1/CD95 (Fas) monoclonal antibody ARG098 on RA synoviocytes and infiltrating lymphocytes, but not on normal chondrocytes (Tamburstuen, 2010)
Normal Chondrocytes were also utilized in a functional cluster formation agarose assay that allows chondrocytes to maintain their differentiated state (Quintavalla, 2005) and to investigate integrin-mediated mechanotransduction pathways required for proper chondrocyte function (Whitney, 2012). Additionally, they have used extensively in material studies aimed to improve chondrocyte adhesion to medical implants (Gutwein, 2002; Ellison, 2003; Price, 2004; Savaiano, 2004; Burns, 2009) and scaffolds for cartilage regeneration (Jun, 2002; Kay, 2002; Miller, 2002a,b; Price, 2002, 2003; Rao, 2004; Park, 2005; Khang, 2008). Normal chondrocyte RNA was used as a gold standard control in research on cellular reprogramming into chondrocytes (Ishii, 2012).
細胞株の由来
Cartilage
アプリケーション
production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration
構成
Basal Medium containing 10% FBS & 10% DMSO
調製ノート
- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
継代と培養方法
Please refer to the HC Culture Protocol.
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
402-05F:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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