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詳細
Poly-D-Lysine (PDL) and Laminin solution is ideal for use in the coating protocol for cell culture surfaces. PDL Laminin coating is extensively used for neural cultures. It is known to enhance cell adhesion and cell maturation during in vitro cell culture. Poly-D-Lysine and Laminin solution is prepared in phosphate-buffered saline and sterile filtered. It is used to coat tissue culture ware to culture surface ratio of 0.25 ml/cm2. Tissue culture ware is coated at room temperature for one hour and carefully aspirate the solution. After wash two times with PBS, coated tissue culture ware may be used immediately or air-dried and stored at 4oC for up to one week.
アプリケーション
Poly-D-Lysine & Laminin (2.5 ML) has been used in vitro as inductive agents to stimulate Y79 cells to express both neuronal and glial characteristics.It has been used to coat culture plates for retinal ganglion cell culture.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
127-2.5:
試験成績書(COA)
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Investigative ophthalmology & visual science, 30(8), 1700-1707 (1989-08-01)
Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been
Journal of molecular neuroscience : MN, 60(4), 486-497 (2016-08-31)
Bone marrow-derived mesenchymal stem cells (MSCs) are the ideal transplanted cells of cellular therapy for promoting neuroprotection and neurorestoration. However, the optimization of transplanted cells and the improvement of microenvironment around implanted cells are still two critical challenges for enhancing
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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