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Merck

B3051

Millipore

Brucella broth base

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSCコード:
41171607
NACRES:
NA.85

無菌性

non-sterile

品質水準

形状

powder

シェルフライフ

limited shelf life, expiry date on the label

メーカー/製品名

NutriSelect® Plus

テクニック

microbiological culture: suitable

pH

7.0±0.2 (25 °C)

アプリケーション

clinical testing
environmental
food and beverages
sample preparation

microbiology

適合性

selective for Brucella spp.
selective for Campylobacter spp.

アプリケーション

Recommended for enrichment, isolation and cultivation of Brucella species.

構成

Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium bisulphite 0.10

調製ノート

Suspend 14.05 g of Brucella Broth Base in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 5% v/v heat inactivated (H1138) horse serum. Add polymyxin B (2,500 IU, Cat. No. 81334), bacitracin (12,500 IU, Cat. No. 11702), nystatin (50,000 IU, Cat. No. 74721), cycloheximide (50mg, Cat. No. 01810), nalidixic acid (2.5mg, Cat. No. 70162) and vancomycin (10mg, Cat. No. 94747) to make the medium selective. Mix well before pouring into sterile petri plates or tubes.
For isolation of the Campylobacter species add the rehydrated contents of one vial of Campylobacter Growth Supplement (Cat. No. C2847) and 5-7% defibrinated sheep blood to 500 ml of medium base. 500 ml of medium base. The Campylobacter Growth Supplement can also be made by mixing 125 mg sodium pyruvate (Cat. No. 15990), 125 mg sodium metabisulfite (Cat. No. 71928 )and 125 mg ferrous sulfate (Cat. No. 44970) in 2 ml of distilled water and filtering the solution through a sterile 0.4 mm filter to sterilize.

脚注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法的情報

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

11 - Combustible Solids

WGK

WGK 3

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

B3051-VAR:
B3051-500G:
B3051-BULK:


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Slide 1 of 3

1 of 3

Mohsen Tabasi et al.
Comparative immunology, microbiology and infectious diseases, 63, 87-93 (2019-04-10)
Brucellosis is the most common zoonotic diseases worldwide. The aim of this study was to develop and evaluate the diagnostic performance of an indirect-ELISA (I-ELISA) method based on whole cell Brucella abortus S99 lysates for detection of IgM anti-Brucella antibodies
Carla Palacios-Gorba et al.
Food & function, 11(5), 4525-4534 (2020-05-13)
Currently, Helicobacter pylori is the unique biological carcinogenic agent. The search for antimicrobial alternatives to antibiotics against this pathogen has been categorized as a priority due to the drastic failure associated with current applied antibiotic therapy. The present study assessed
A selective medium for the isolation of brucellae from contaminated materials.
C D KUZDAS et al.
Journal of bacteriology, 66(4), 502-504 (1953-10-01)
A preliminary report on a selective medium for the culture of Brucella, including fastidious types.
L M JONES et al.
Bulletin of the World Health Organization, 19(1), 200-203 (1958-01-01)
[A selective medium for isolation of Brucella melitensis].
G RENOUX
Annales de l'Institut Pasteur, 87(3), 325-333 (1954-09-01)

資料

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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