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詳細
KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
アプリケーション
KAPA2G Fast HotStart® PCR Kit has been used in:
- Real time PCR
- Routine PCR
- Fast PCR
- Genotyping
生物化学的／生理学的作用
KAPA2G Fast DNA Polymerase generated DNA fragments may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
特徴および利点
Reduce cycling times up to 75% :
High speed, higher performance :
Quick Notes :
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes :
- KAPA2G Fast HotStart PCR Kits with dNTPs contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.Optimized buffer system offers improved yields,specificity and sensitivity, facilitating efficient primerannealing across a wide range of primer lengths,GC contents, and melting temperatures.
- Use 0.5 U KAPA2G Fast HotStart DNA Polymerase per 25 μL reaction, or less for smaller volumes.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.The buffer is available with and without MgCl2 KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X, while KAPA2G Buffer M is Mg-free. Aside from MgCl2 content, these two buffers are identical.Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
品質
Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
調製ノート
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
その他情報
For Research Use Only. Not for use in diagnostic procedures.
法的情報
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
キットの構成要素のみ
製品番号
詳細
- KAPA2G Fast Standard or HotStart® DNA Polymerase 5 U/μL
- 5X KAPA2G Buffer A with MgCl2
- MgCl2 25 mM
- dNTP Mix 10 mM each dNTP
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
does not flash
引火点(℃)
does not flash
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Yunlong Zhao et al.
Journal of clinical laboratory analysis, 33(2), e22670-e22670 (2018-09-08)
The ratio of target cfDNA in total plasma is low. The abundant gDNA background resulting from blood cell lysis caused by improper operation has become a major obstacle to accurately measure cfDNA. In this study, we investigated the storage capacity
Francesca Del Bufalo et al.
Frontiers in oncology, 8, 526-526 (2018-11-30)
Low-grade gliomas (LGG) are the most common central nervous system tumors in children. Prognosis depends on complete surgical resection. For patients not amenable of gross total resection (GTR) new approaches are needed. The BRAF mutation V600E is critical for the
資料
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
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