11759779001

BM Chemiluminescence ELISA Substrate (AP)

• UNSPSCコード: 12352204

特性

• 使用法: sufficient for 1,000 tests (ELISA); sufficient for 600 tests (SEAP)
• 包装: pkg of 150 mL
• メーカー／製品名: Roche
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

特異性

The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

特徴および利点

• Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
• High dynamic measuring range: Linear range of more than five orders of magnitude
• Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

構成

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer

原理

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.

調製ノート

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

その他情報

For life science research only. Not for use in diagnostic procedures.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): does not flash
• 引火点(℃): does not flash