詳細
Designed to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
This Western-Re-Probe Reagent is validated for use in Immunoblotting.
Western-Re-Probe Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.
アプリケーション
Immunoblotting (see comments)
構成
5X Re-Probe solution (125 ml)
警告
Toxicity: Standard Handling (A)
物理的形状
Supplied as a 5X solution.
その他情報
Sufficient volume to strip 25-30 standard blots.
ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes
PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe in water.
2- Incubate the membrane in 1X Western-Re-Probe buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.
Western-Re-Probe Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.
PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetection
ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes
PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe in water.
2- Incubate the membrane in 1X Western-Re-Probe buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.
Western-Re-Probe Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.
PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetection
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
WB59-ML:
WB59-100ML:
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