SCYT-91K-PXBK14

Ovine Cytokine/Chemokine Panel 1 14 Plex

Immuno-Oncology Multiplex Assay

• 別名: 14 Plex Chemokine Panel, Ovine Cytokine Assay, Ovine Cytokine Panel
• UNSPSCコード: 12161503
• NACRES: NA.47

特性

• 化学種の反応性: mouse
• 包装: pkg of 1 ea
• メーカー／製品名: Milliplex^®
• assay range: accuracy: 101; (Galectin-1); accuracy: 102; (PD-L2); accuracy: 103; (CD80/B7-1); accuracy: 103; (IFNγ); accuracy: 104; (CTLA-4/CD152); accuracy: 104; (TLR-2); accuracy: 105; (4-1BBL/TNFSF9); accuracy: 105; (BCA-1/CXCL-13); accuracy: 105; (CD25/IL-2Rα); accuracy: 105; (IL-10); accuracy: 105; (LAG-3); accuracy: 106; (5’-NT/CD73); accuracy: 106; (PD-1); accuracy: 106; (TIM-3); accuracy: 106; (TNFα); accuracy: 107; (CD137/4-1BB); accuracy: 107; (CD27); accuracy: 108; ( Galectin-3); accuracy: 108; (E-Cadherin); accuracy: 108; (HVEM); accuracy: 109; (CD40); accuracy: 109; (GITR); accuracy: 110; (B7-H2/ICOSL); accuracy: 110; (CD226/DNAM-1); accuracy: 111; (BTLA); accuracy: 94; (GranzymeB); accuracy: 95; (B7-H3/CD276); accuracy: 96; (PD-L1); sensitivity: 14 - 10000 pg/mL; (4-1BBL/TNFSF9); standard curve range: 1.4 - 1000 pg/mL; (HVEM); standard curve range: 1.4 - 1000 pg/mL; (IFNγ); standard curve range: 11 - 8000 pg/mL; (IL-10); standard curve range: 137 - 100000 pg/mL; (5’-NT/CD73); standard curve range: 14 - 10000 pg/mL; (CD226/DNAM-1); standard curve range: 14 - 10000 pg/mL; (CD40); standard curve range: 14 - 10000 pg/mL; (PD-L1); standard curve range: 2.7 - 2000 pg/mL; (CD25/IL-2Rα); standard curve range: 21 - 15000 pg/mL; (BTLA); standard curve range: 27 - 20000 pg/mL; (B7-H2/ICOSL); standard curve range: 27 - 20000 pg/mL; (BCA-1/CXCL-13); standard curve range: 3.4 - 2500 ng/mL; (Galectin-1); standard curve range: 3.4 - 2500 ng/mL; (B7-H3/CD276); standard curve range: 34.3 - 25000 pg/mL; (CD80/B7-1); standard curve range: 342 - 250000 pg/mL; (E-Cadherin); standard curve range: 4.1 - 3000 pg/mL; (TNFα); standard curve range: 41 - 30000 pg/mL; (GITR); standard curve range: 41 - 30000 pg/mL; (GranzymeB); standard curve range: 41 - 30000 pg/mL; (Galectin-3); standard curve range: 412 - 300000 pg/mL; (LAG-3); standard curve range: 412 - 300000 pg/mL; (TLR-2); standard curve range: 5.5 - 4000 pg/mL; (CD137/4-1BB ); standard curve range: 5.5 - 4000 pg/mL; (CTLA-4/CD152); standard curve range: 5.5 - 4000 pg/mL; (TIM-3); standard curve range: 55 - 40000 pg/mL; (CD27); standard curve range: 55 - 40000 pg/mL; (PD-1); standard curve range: 6.9 - 5000 pg/mL; (PD-L2); inter-assay cv: <20%; intra-assay cv: <10%
• テクニック: multiplexing: suitable
• 適合性: configured for Premixed
• 検出方法: fluorometric (Luminex^® xMAP^®)
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Immune checkpoint proteins are key regulators of the immune system and drug targets of cancer immunotherapy. Immune checkpoint molecules have been found on the cell surface of immune cells and tumor cells as activating receptors or inhibitory receptors. Immune checkpoint inhibitors, such as blocking antibodies to cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), programmed cell death protein-1 (PD-1) and programmed cell death ligand 1 (PD-L1), have demonstrated significant efficacy in the treatment of a variety of cancers. Recent studies have also shown many immune checkpoint proteins can undergo alternative splicing or proteolytic cleavage to produce soluble checkpoint proteins. The expression levels of soluble and cell-surface immune checkpoint proteins in the circulation and in tumor-microenvironment (TME) may be used as putative immune-based biomarkers for cancer research and biomarker discovery in immunological diseases.
MILLIPLEX^® Mouse Immuno-Oncology Checkpoint Protein Panel 1 is a 28-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples: 4-1BBL/TNFSF9, 5′-NT/CD73, B7-H2/ICOSL, B7-H3/CD276, BCA-1/CXCL-13, BTLA, CD137/4-1BB, CD226/DNAM-1, CD25/IL-2Rα, CD27, CD40, CD80/B7-1, CTLA-4/CD152, E-cadherin, Galectin-1, Galectin-3, GITR, Granzyme B, HVEM, IFNγ, IL-10, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2, and TNFα.

Panel Type: MAGNETIC Circulating Cancer

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

アプリケーション

The Mouse Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex^® xMAP^® technology, enables the simultaneous analysis of 28 immune checkpoint proteins in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.
This is an overnight incubation assay (16-18 hours at 2-8ºC).
A maximum of 25 μL per well 1:2 diluted serum or plasma can be used.
Tissue culture samples may be run neat or appropriately diluted in control medium.
Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.

Research Category: Cancer/Immuno-oncology

Analytes:
4-1BBL/TNFSF9
5’-NT/CD73
B7-H2/ICOSL
B7-H3/CD276
BCA-1/CXCL-13
BTLA
CD137/4-1BB
CD226/DNAM-1
CD25/IL-2Rα
CD27
CD40
CD80/B7-1
CTLA-4/CD152
E-cadherin
Galectin-1
Galectin-3
GITR
Granzyme B
HVEM
IFNγ
IL-10
LAG-3
PD-1
PD-L1
PD-L2
TIM-3
TLR-2
TNFα

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

包装

96-well plate

その他情報

Sensitivity: Refer to kit protocol for sensitivities for individual cytokines/chemokines.

法的情報

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08,GHS05,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H312 + H332,H317,H318,H373,H411
• 注意書き: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危険有害性の分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• ターゲットの組織: Respiratory Tract
• 保管分類コード: 10 - Combustible liquids