テクニック
cell based assay: suitable
検出方法
fluorometric
輸送温度
ambient
関連するカテゴリー
詳細
Pluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). Characterization of human iPS cells includes alkaline phosphatase and antibody staining for Oct-4, Sox-2, Nanog, SSEA and TRA surface markers. These assays require cell fixation and are end-point assays.
The BioTracker 529 Green Pluripotent Stem Cell Dye is a live stem cell probe that selectively identifies undifferentiated human ES and iPS cells. The probe localizes at mitochondria in the human stem cells. The dye’s fluorescence is dependent on the activities of ABCB1 and ABCG2 on the cell membrane. The dye is suitable for both live cell imaging and flow cytometry applications of human pluripotent stem cells.
Spectral Properties
Absorbance: 515nm
Emission: 529nm
The BioTracker 529 Green Pluripotent Stem Cell Dye is a live stem cell probe that selectively identifies undifferentiated human ES and iPS cells. The probe localizes at mitochondria in the human stem cells. The dye’s fluorescence is dependent on the activities of ABCB1 and ABCG2 on the cell membrane. The dye is suitable for both live cell imaging and flow cytometry applications of human pluripotent stem cells.
Spectral Properties
Absorbance: 515nm
Emission: 529nm
アプリケーション
Live cell fluorescent imaging
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
物理的形状
Lyophilized
保管および安定性
Store BioTracker 529 Green Pluripotent Stem Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
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