S7821-M
CpGenome Universal Methylated DNA
Enzymatically methylated human male genomic DNA to be used as a methylation-positive control for gene methylation studies.
別名:
Enzymatically methylated human male genomic DNA, Methylation-positive control
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About This Item
UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.32
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詳細
CpGenome Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.
Materials Provided:
Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.
Validation:
Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Materials Provided:
Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.
Validation:
Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
物理的形状
Liquid in TE (10mM Tris-HCL, 0.1mM EDTA) with no preservatives.
保管および安定性
Recommended Storage: -15°C to -25°C, in aliquots, protected from freeze-thaws for up to two years from date of receipt. Do not freeze and thaw the same -20°C aliquot more than 3X for best results. Multiple freeze thaws can fragment DNA. Storage at -70°C can be used for longer term storage if necessary; minimize multiple freeze thaws for best results.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
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Methylation Status of the O6-Methylguanine-Deoxyribonucleic Acid Methyltransferase Gene Promoter in World Health Organization Grade III Gliomas.
Seung-Heon Yang,Yong Hwy Kim,Jin Wook Kim,Chul-Kee Park,Sung-Hye Park,Hee-Won Jung
Journal of Korean Neurosurgical Society null
Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.
Victoria L Boyd, Gerald Zon
Analytical biochemistry null
Shinobu Sato et al.
Analytica chimica acta, 578(1), 82-87 (2007-08-29)
Ferrocenylnaphthalene diimide (FND)-based electrochemical hybridization assay was applied to the detection of methylated cytosine of DNA using the products obtained after treatment with bisulfite followed by polymerase chain reaction (PCR), where unmethylated cytosine is converted to thymine and methylated one
Closed-tube PCR methods for locus-specific DNA methylation analysis.
Ida L M Candiloro,Thomas Mikeska,Alexander Dobrovic
Methods in Molecular Biology null
Detection of epigenetic changes in fecal DNA as a molecular screening test for colorectal cancer: a feasibility study
Leung, Wai K, et al
Clinical Chemistry, 50, 2179-2182 (2004)
資料
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it is to identify one topic that is completely unrelated to any other.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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