S8001M
CpGenome Human Methylated DNA Standard Set
It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
別名:
CpGenome Methylated DNA, Human DNA Standards, Methylated DNA Standard Set
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About This Item
UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.77
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詳細
The CpGenome Human Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. This Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase. It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ amplification kits.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
物理的形状
Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
保管および安定性
Recommended Storage: Stable at -20°C for up to 6 months from date of receipt.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
引火点(°F)
does not flash
引火点(℃)
does not flash
適用法令
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Jan Code
S8001M:
試験成績書（COA）
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