QIA92

Caspase Detection Kit (Red-VAD-FMK)

• UNSPSCコード: 41116133

特性

• 使用法: sufficient for 100 tests
• 品質水準: 100
• 交差性（ホモロジーによる予測）: mammals
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: flow cytometry: suitable
• 入力: sample type intact cells
• 蛍光検出: λ_ex ~540 nm; λ_em ~570 nm
• 検出方法: fluorometric
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker is VAD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader or flow cytometry.

The activation of caspases plays a central role in apoptosis. The Caspase Detection Kit provides a convenient and sensitive means for detecting activated caspases in situ in living cells. The assay utilizes a caspase inhibitor (VAD-FMK) conjugated to sulfo-rhodamine as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells. The red fluorescence label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

特異性

A broad range of species

構成

Labeled Caspase Inhibitor (Red-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

規格

Assay Time: 1.5 h

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids