PRCYT2MAG40K

MILLIPLEX^® Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

• UNSPSCコード: 12161503
• eCl@ss: 32161000

特性

• 品質水準: 200
• 化学種の反応性: nonhuman primates
• メーカー／製品名: Milliplex^®
• assay range: accuracy: 80-109%; (Overnight Incubation); standard curve range: 1-1,500 pg/mL; (IL-17A); standard curve range: 1-750 pg/mL; (RANTES/CCL5 (tissue culture)); standard curve range: 10-10,000 pg/mL; (sCD137, IL-2, IP-10/CXCL10); standard curve range: 146-150,000 pg/mL; (IL-4); standard curve range: 15-15,000 pg/mL; (IL-21, IL-31); standard curve range: 20-20,000 pg/mL; (FGF-2, IL-33); standard curve range: 24-25,000 pg/mL; (IL-23); standard curve range: 29-30,000 pg/mL; (IL-1α, IL-28A); standard curve range: 293-300,000 pg/mL; (IL-17E/IL-25); standard curve range: 3-3,000 pg/mL; (Eotaxin/CCL11, IL-6); standard curve range: 4-4,000 pg/mL; (Granzyme B); standard curve range: 7-7,000 pg/mL; (MIP-3α/CCL20); standard curve range: 73-75,000 pg/mL; (IL-16); standard curve range: 78-80,000 pg/mL; (Granzyme A); standard curve range: 8-8,000 pg/mL; (sFasL); standard curve range: 98-100,000 pg/mL; (Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA)
• テクニック: multiplexing: suitable
• 検出方法: fluorometric (Luminex xMAP)
• 輸送温度: wet ice

詳細

詳細

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

The MILLIPLEX^® Non-Human Primate Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of any or all of the following analytes in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

アプリケーション

• Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA
• Recommended Sample type: Serum, plasma, or cell culture supernatant
• Recommended Sample dilution: 1:2 diluted plasma or serum
• NOTE: RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs
• Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended
• Research Category: Inflammation & Immunology

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

構成

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized

Sample Plate, 96 well format, 1 plate

2 Foil plate sealers

Assay Buffer, 2 bottles, 30 mL

Bead Diluent, 1 bottle, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

その他情報

Please contact Technical Service for linearity of dilution.

Please note: Sample Type must be selected when configuring this kit. RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes for serum/plasma.

Sensitivity: Refer to kit protocol for sensitivities for individual cytokines/chemokines

法的情報

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

安全性情報

• ピクトグラム: GHS05,GHS06,GHS08,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H332 - H311 - H317 - H318 - H373 - H411
• 注意書き: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危険有害性の分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• ターゲットの組織: Respiratory Tract
• 保管分類コード: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3