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Merck

PC177L

Sigma-Aldrich

Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

lyophilized, Calbiochem®

別名:

Rabbit Anti-Serotonin Transporter, Serotonin Transporter Antibody

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.43

由来生物

rabbit

品質水準

抗体製品の状態

serum

抗体製品タイプ

primary antibodies

クローン

polyclonal

形状

lyophilized

含みます

≤0.1% sodium azide as preservative

化学種の反応性

mouse, rat

メーカー/製品名

Calbiochem®

保管条件

OK to freeze
avoid repeated freeze/thaw cycles

アイソタイプ

IgG

輸送温度

ambient

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

mouse ... Slc6A4(15567)

詳細

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

免疫原

Rat
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter

アプリケーション


Frozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)

警告

Toxicity: Standard Handling (A)

物理的形状

Undiluted serum.

再構成

Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.

アナリシスノート

Positive Control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord

その他情報

Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

10 - Combustible liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

PC177L-100UL:
PC177L-UL:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

S Nullmeier et al.
The European journal of neuroscience, 33(4), 689-698 (2011-01-14)
Heterozygous reeler mice (HRM), haploinsufficient for reelin, have been proposed to be a genetic mouse model of schizophrenia. Beside behavioural similarities, HRM also demonstrate several neuroanatomical traits similar to patients suffering from schizophrenia. In the present study using immunocytochemical procedures
Diana K Sarko et al.
The Journal of comparative neurology, 519(1), 64-74 (2010-12-02)
Northern grasshopper mice (Onychomys leucogaster) are among the most highly carnivorous rodents in North America. Because predatory mammals may have specialization of senses used to detect prey, we investigated the organization of sensory areas within grasshopper mouse neocortex and quantified
Andrea Forero et al.
Frontiers in cellular neuroscience, 11, 307-307 (2017-10-12)
Background: During early prenatal stages of brain development, serotonin (5-HT)-specific neurons migrate through somal translocation to form the raphe nuclei and subsequently begin to project to their target regions. The rostral cluster of cells, comprising the median and dorsal raphe
Rebecca L Lowery et al.
International journal of molecular sciences, 22(4) (2021-03-07)
While microglia have been established as critical mediators of synaptic plasticity, the molecular signals underlying this process are still being uncovered. Increasing evidence suggests that microglia utilize these signals in a temporally and regionally heterogeneous manner. Subsequently, it is necessary
R Ye et al.
Genes, brain, and behavior, 13(3), 261-275 (2014-01-11)
Presynaptic serotonin (5-hydroxytryptamine, 5-HT) transporters (SERT) regulate 5-HT signaling via antidepressant-sensitive clearance of released neurotransmitter. Polymorphisms in the human SERT gene (SLC6A4) have been linked to risk for multiple neuropsychiatric disorders, including depression, obsessive-compulsive disorder and autism. Using BXD recombinant

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