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Merck

MSIPN4W50

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, non-sterile

別名:

PVDF filter plate

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About This Item

UNSPSCコード:
41104923
eCl@ss:
32039006
NACRES:
NB.22

物質

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

品質水準

詳細

Non-sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 50.

無菌性

non-sterile

製品種目

MultiScreen®

特徴

hydrophobic
lid

メーカー/製品名

MultiScreen®

パラメーター

50-250 μL sample volume (per well)

テクニック

ELISA: suitable
ELISPOT: suitable
activity assay: suitable (protein interaction)

ろ過面積

0.28 cm2

プレートサイズ

96 wells

ウェルの最大容量

300 μL

有効容積

50-250 μL

マトリックス

Immobilon®-P

ポアサイズ

0.45 μm pore size

結合型

high binding surface

輸送温度

ambient

詳細

Filter Code: IP
No matter what application you’re working with, Millipore has a plate developed to meet your specific needs. MultiScreen plates have a long history as a reliable tool for the advancement of drug discovery and life science research.

HTS Filter Plates (Plate catalogue numbers that start with MS)
- MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.

- Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.

Classic Filter Plates (Plate catalogue numbers that start with MA)
- The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.

Accessory – MultiScreenHTS Vacuum Manifold
- The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.

Millicell Assay Plates
- In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.

アプリケーション

Research Category
Assays

Protein

Radionucleotides

物理的形状

Color Code: 白色

その他情報

Automation Compatible: Yes

法的情報

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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試験成績書(COA)

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Brian T Gaudette et al.
Nature communications, 11(1), 723-723 (2020-02-07)
How activated B cells build biosynthetic pathways and organelle structures necessary for subsequent robust antibody secretion is still unclear. The dominant model holds that nascent plasma cells adapt to increased antibody synthesis by activating the unfolded protein response (UPR) under

資料

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

This article provides white paper on Elispot assays: state-of-the-art tools for functional analysis of cellular immunology.

プロトコル

This page shows an example protocol of an ELISpot assay for quantifying the cells producing interferon-gamma in response to antigen or non-specific activation using phytohemagglutinin.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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