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物質
PVDF membrane (hydrophobic)
acrylic
flat bottom wells
品質水準
詳細
Non-sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 50.
無菌性
non-sterile
製品種目
MultiScreen®
特徴
hydrophobic
lid
メーカー/製品名
MultiScreen®
パラメーター
50-250 μL sample volume (per well)
テクニック
ELISA: suitable
ELISPOT: suitable
activity assay: suitable (protein interaction)
ろ過面積
0.28 cm2
プレートサイズ
96 wells
ウェルの最大容量
300 μL
有効容積
50-250 μL
マトリックス
Immobilon®-P
ポアサイズ
0.45 μm pore size
結合型
high binding surface
輸送温度
ambient
詳細
HTS Filter Plates (Plate catalogue numbers that start with MS)
- MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.
- Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.
Classic Filter Plates (Plate catalogue numbers that start with MA)
- The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.
Accessory – MultiScreenHTS Vacuum Manifold
- The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.
Millicell Assay Plates
- In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.
アプリケーション
Assays
Protein
Radionucleotides
物理的形状
その他情報
法的情報
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試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
This article provides white paper on Elispot assays: state-of-the-art tools for functional analysis of cellular immunology.
プロトコル
This page shows an example protocol of an ELISpot assay for quantifying the cells producing interferon-gamma in response to antigen or non-specific activation using phytohemagglutinin.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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