MABT529

Anti-Integrin beta 1 (CD29) Antibody

mouse monoclonal, 13C4.1

• 別名: Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 製品名: Anti-Integrin beta-1 (CD29) Antibody, clone 13C4.1, clone 13C4.1, from mouse
• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 13C4.1, monoclonal
• 交差性: human
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1λ
• NCBIアクセッション番号: NP_002202
• UniProtアクセッション番号: P05556
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ITGB1(3688)

詳細

詳細

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

特異性

Clone 13C4.1 targets an epitope within the membrane-proximal extracellular region present in all five spliced isoforms of human Integrin beta-1 reported by UniPort (P05556).

免疫原

GST-tagged recombinant human Integrin beta-1 fragment corresponding to the membrane-proximal extracellular region.

アプリケーション

Research Category
細胞骨格

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Integrin beta-1 in human breast cancer, cerebral cortex, and lung tissue sections.

This mouse monoclonal Anti-Integrin beta-1 (CD29), clone 13C4.1, Cat. No. MABT529, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of Integrin beta-1.

品質

Evaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Integrin beta-1 in 10 µg of U937 cell lysate.

ターゲットの説明

~150 kDa observed. Target band size appears larger than the calculated molecular weights of 88.42/87.45/91.62/91.03/88.88 kDa (isoform Beta-1A/Beta-1B/Beta-1C/Beta-1C-2/Beta-1D pro-form) and 86.19/85.22/89.40/88.81/86.66 kDa (mature isoform Beta-1A/Beta-1B/Beta-1C/Beta-1C-2/Beta-1D) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

関連事項

Replaces: 04-1109

物理的形状

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable