MABT1558

Anti-Utrophin Antibody, clone MANCHO3 (8A4)

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: MANCHO3 (8A4), monoclonal
• 分子量: calculated mol wt 394.47 kDa; observed mol wt ~400 kDa
• 精製方法: using protein G
• 化学種の反応性: human, mouse
• 化学種の反応性（ホモロジーによる予測）: canine
• 包装: antibody small pack of 100 μg
• テクニック: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_009055
• UniProtアクセッション番号: P46939
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... UTRN(7402)

詳細

詳細

Utrophin (UniProt: P46939; also known as Dystrophin-related protein 1, DRP-1) is encoded by the UTRN (also known as DMDL, DRP1) gene (Gene ID: 7402) in human. Utrophin is a dystrophin related protein that displays a sequence homology with dystrophin and shares many of the protein-binding properties. It plays a role in anchoring the cytoskeleton to the plasma membrane. It contains 2 calponin homology domains in its N-terminal region (aa 31-135 and 150-255) and has 22 spectrin homology repeats. The C-terminal sequence in utrophin is highly conserved between dystrophin and utrophin in most species. Utrophin is distributed throughout the sarcolemma in fetal and regenerating muscle, but is shown to be down-regulated in normal adult muscle and is restricted to the myotendinous and neuromuscular junctions. Four different isoforms of utrophin have been described that are produced by alternative promoter usage and they differ in their distribution pattern. Isoform 1 displays high expression in muscle tissue. Similar to dystrophin, utrophin can also reported to form an extensive lateral association with actin filaments and protect actin filaments from depolymerization in vitro. However, utrophin binds laterally along actin filaments through contribution of acidic spectrin-like repeats rather than the cluster of basic repeats used by dystrophin. It has been suggested that utrophin could functionally compensate for the lack of dystrophin and studied have shown that transgenic overexpression of utrophin in dystrophin-deficient mdx mice results in full recovery for all known parameters of the dystrophic phenotype. (Ref.: Sengupta, K., et al. (2020). Sci. Rep. 10; 21492; Porter, JD., et al.(1998). J. Cell Sci. 111(13); 1801-1811; Rybakova, IN., et al., (2002). Mol. Biol. Cell. 13(5); 1512-1521).

特異性

Clone MANCHO3 (8A4) is a mouse monoclonal antibody that detects Utrophin. It targets an epitope within 42 amino acids from the C-terminal region.

免疫原

A recombinant fragment corresponding the 329 amino acids from the C-terminal region of human Utrophin.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Utrophin in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Utrophin in NIH3T3 cell lysate.

Immunofluorescence Analysis: A representative lot detected Utrophin in Immunofluorescence applications (McMorran, B.J., et al. (2016). Glycobiology. 26(10):1120-1132).

Immunohistochemistry Applications: A representative lot detected Utrophin in Immunohistochemistry applications (Nguyen T.M. et al. (1991). J Cell Biol. 115(6):1695-700).

Western Blotting Analysis: A representative lot detected Utrophin in Western Blotting applications (Morris, G.E., et al. (1998). Biochemistry. 37(31):11117-27; Nguyen T.M. et al. (1991). J Cell Biol. 115(6):1695-700; Lien, C.F., et al. (2012). J Biol Chem. 287(49):41374-85; Ghosh, T., et al. (2015). PLoS One;10(7):e0134809; Lindsay, A., et al. (2019). FEBS J.;286(13):2562-2576).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Utrophin, clone MANCHO3 (8A4), Cat. No. MABT1558, is a mouse monoclonal antibody that detects Utrophin and is tested for use in Immunofluorescence, , Immunohistochemistry, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable