MABT1330

Anti-ADAM 12 Antibody, clone 8F8

clone 8F8, from mouse

• 別名: Disintegrin and metalloproteinase domain-containing protein 12, Meltrin-alpha
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 8F8, monoclonal
• application: FACS; ICC; IP; WB; inhibition assay
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable

特性

• 由来生物: mouse
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 8F8, monoclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μg
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001275903
• UniProtアクセッション番号: O43184
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ADAM12(8038)

詳細

詳細

Disintegrin and metalloproteinase domain-containing protein 12 (UniProt: O43184, also known as ADAM 12, Meltrin-alpha) is encoded by the ADAM12 (also known as MLTN, UNQ346/PRO545) gene (Gene ID: 8038) in human. ADAM (A disintegrin and metalloprotease) family consists of multidomain cell-surface proteins that are synthesized in their proform. Four isoforms of ADAM12 have been described that are produced by alternative splicing. Isoform 1 is a single-pass transmembrane protein and isoforms 2,3, and 4 are secreted proteins. ADM12 is synthesized with a signal peptide (aa 1-28) and a propeptide (aa 29-207), which are subsequently cleaved off in the mature form. Isoform 1 shown to be expressed in placenta and in skeletal, cardiac, and smooth muscle. Isoform 2 is reported to be expressed only in placenta or in embryo and fetus. ADAM12 contains a cysteine rich domain (aa 514-649) that support cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. However, in carcinoma cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. Dissociation of the cysteine from the zinc ion activates the enzyme. Upregulation of ADAM12 has been reported in malignant tumors. In human breast cancer cells expressing ADAM12, MMP-14 is shown to be recruited to the cell surface which results in ADAM12 activation and accelerates tumor progression. ADAM12 does not affect tumor cell proliferation, but it causes a diminution in tumor cell apoptosis and increases stromal cell apoptosis. (Ref.: Sundberg, C., et al. (2004). J. Biol. Chem. 279(49); 51601 51611; Albrechtsen, R., et al. (2013). J. Cell Sci. 126(20); 4707 4720; Kveiborg, M., et al. (2005). Cancer Res. 65(11); 4754-4761).

特異性

Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.

免疫原

A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.

アプリケーション

Research Category
アポトーシス及び癌

Anti-ADAM 12, clone 8F8, Cat. No. MABT1330, is a mouse monoclonal antibody that detects Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, and Western Blotting.

Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).

Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20).

Flow Cytometry Analysis: 1 µg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.

Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).

Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).

品質

Evaluated by Immunocytochemistry in ADAM 12-DeltaCyt MCF-7 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected ADAM 12-DeltaCyt MCF-7 cells.

ターゲットの説明

99.54 kDa calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable