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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
4F1, monoclonal
交差性
rat, mouse, human
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG2a
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... UBD(10537)
詳細
FAT10 protein is a recombinant protein produced in E. coli corresponding to the human sequence encompassing an N-terminal His6-tag within a leader sequence. FAT10, a ubiquitin-like protein, is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. FAT10 expression causes apoptosis and it appears that FAT10 may modulate tumorigenesis through its interaction with the MAD2 spindle-assembly checkpoint protein. It has been demonstrated that FAT10 and its conjugates are rapidly degraded by the proteasome. Furthermore, a new interaction partner of FAT10, NEDD8 ultimate buster-1L (NUB1L), may function as a linker that targets FAT10 for degradation by the proteasome.
免疫原
GST-tagged recombinant protein corresponding to human FAT10.
アプリケーション
Anti-FAT10 Antibody, clone 4F1 is a highly specific mouse monoclonal antibody, that targets FAT10 & has been tested in western blotting, IP & ICC.
Immunocytochemistry Analysis: A representative lot detected FAT10 in HA-FAT10 transfected HEK293 cells (Aichem, A., et al. (2010). Nature Commun. 1:13).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10 in transfected HA-FAT10 HEK293 cells cultivated with IFNgamma and TNFalpha (Aichem, A., et al. (2010). Nature Commun. 1:13).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10 in transfected HA-FAT10 HEK293 cells cultivated with IFNgamma and TNFalpha (Aichem, A., et al. (2010). Nature Commun. 1:13).
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAT10 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAT10 in 10 µg of HepG2 cell lysate.
ターゲットの説明
~18 kDa observed. FAT10 FAT10ylates itself as well as other proteins so additional bands may be observed due to the FAT10ylation of endogenous proteins (Aichem, A., et al. (2010). Nature Commun. 1:13.),(Aichem, A., et al. J Cell Sci. (2012)125:4576-85).
物理的形状
Format: Purified
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS351:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Annette Aichem et al.
Journal of cell science, 125(Pt 19), 4576-4585 (2012-07-17)
FAT10 is a ubiquitin-like modifier proposed to function in apoptosis induction, cell cycle control and NF-κB activation. Upon induction by pro-inflammatory cytokines, hundreds of endogenous substrates become covalently conjugated to FAT10 leading to their proteasomal degradation. Nevertheless, only three substrates
Annette Aichem et al.
Nature communications, 1, 13-13 (2010-10-27)
The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction partner of FAT10. Activated FAT10 can be transferred
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