MABS1925

Anti-carboxypeptidase M Antibody, clone 7F9

clone 7F9, from mouse

• 別名: CPM, CP-M
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 7F9, monoclonal
• 化学種の反応性: rat
• 包装: antibody small pack of 25 μg
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_001101568.1
• UniProtアクセッション番号: D4A9Q5
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Cpm(314855)

詳細

詳細

Carboxypeptidase M (UniProt: D4A9Q5; also known as CPM) is encoded by the Cpm gene (Gene ID: 314855) in rat. Rat CPM a glycosylphosphatidylinositol (GPI) anchored metallo-carboxypeptidase that cleaves basic carboxyl-terminal amino acids from various peptides at neutral pH. Rat CPM is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form. The mature CPM displays high specificity and cleaves only C-terminal arginine or lysine residues in a variety of peptide substrates. It is shown to be expressed in the lung and placenta at high levels. Its expression is detected in alveolar cells lacking T1alpha, a type I cell marker protein found in adult lung. Carboxypeptidase M also serves as a macrophage differentiation marker, which is preferentially induced in epithelioid cells of all granuloma types studied, but not in resting macrophages. Hence, it can be employed for detection of minute granuloma even in the presence of non-granulomatous macrophages. Clone 7F9 recognizes CPM expressed in late embryonic rat lung to postnatal day 1. (Ref.: Fujiwara, N., et al. (2007), Respirology 12(1); 54-62; Tsakiris, I., et al. (2012). Lab. Invest. 92(3); 345-361).

特異性

Clone 7F9 specifically detects carboxypeptidase M in rat tissues.

免疫原

Epitope: extracellular domain

Membrane fraction of E18.5 embryonic rat lung.

アプリケーション

Research Category
細胞シグナル伝達

Anti-carboxypeptidase M, clone 7F9, Cat. No. MABS1925, is a mouse monoclonal antibody that detects Carboxypeptidase M and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).

Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).

Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).

品質

Evaluated by Immunofluorescence in E19 rat embryo tissue sections.

Immunofluorescence Analysis: A 1:25 dilution of this antibody detected carboxypeptidase M in E19 rat embryo tissue sections.

ターゲットの説明

50.32 kDa calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable