MABS14

Anti-mTOR Antibody, clone F11

clone F11, from mouse

• 別名: FK506 binding protein 12-rapamycin associated protein 1, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated prot
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: F11, monoclonal
• application: IP; WB
• 化学種の反応性: human
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• citations: 1

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: F11, monoclonal
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology)
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_063971
• UniProtアクセッション番号: P42346
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MTOR(2475)

詳細

詳細

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

免疫原

GST-tagged recombinant protein corresponding to rat mTOR.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
PI3K、Akt及びmTORシグナル伝達

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Nemazanyy, I, et al. (2008). Hybridoma, 27:395-398.)

This Anti-mTOR Antibody, clone F11 is validated for use in WB, IP for the detection of mTOR.

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on 10 µg of HeLa cell lysate.

ターゲットの説明

~ 289 kDa observed

物理的形状

Protein G

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable