MABN2709
Anti-LRAT Antibody, clone 49569
別名:
lecithin retinol acyl transferase, retinyl ester synthase
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About This Item
UNSPSCコード:
12352203
NACRES:
NA.43
クローン:
49569, monoclonal
application:
IF
IHC
WB
IHC
WB
化学種の反応性:
mouse
テクニック:
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
49569, monoclonal
分子量
calculated mol wt 25.820 kDa
observed mol wt ~24 kDa
精製方法
using protein G
化学種の反応性
mouse
包装
antibody small pack of 100
テクニック
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG2aκ
エピトープ配列
Cytoplasmic domain
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
2-8°C
遺伝子情報
mouse ... Lrat(79235)
特異性
Clone 49569 is a mouse monoclonal antibody that detects LRAT. It targets an epitope within 91 amino acids from the cytoplasmic domain of the protein.
免疫原
His-tagged recombinant mouse LRAT fragment corresponding to 91 amino acids from the cytoplasmic domain of mouse LRAT.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in mouse retina lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRAT in mouse retina tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRAT in Retinal pigment epithelium (RPE) microsome lysate.
Immunofluorescence Analysis: A representative lot detected LRAT in Immunofluorescence applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Immunohistochemistry Analysis: A representative lot detected LRAT in Immunohistochemistry applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Western Blotting Analysis: A representative lot detected LRAT in Western Blotting applications (Batten, M.L., et al. (2004). J Biol Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J. Biol. Chem. 282(3):2081-90).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in mouse retina lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRAT in mouse retina tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRAT in Retinal pigment epithelium (RPE) microsome lysate.
Immunofluorescence Analysis: A representative lot detected LRAT in Immunofluorescence applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Immunohistochemistry Analysis: A representative lot detected LRAT in Immunohistochemistry applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Western Blotting Analysis: A representative lot detected LRAT in Western Blotting applications (Batten, M.L., et al. (2004). J Biol Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J. Biol. Chem. 282(3):2081-90).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Lecithin retinol acyltransferase (UniProt: Q9JI60) (also known as EC:2.3.1.135) is encoded by the Lrat gene (Gene ID:79235) in mouse. Lecithin-retinol acyltransferase (LRAT) is an essential enzyme involved in the metabolism of retinoids, particularly vitamin A. LRAT contains a conserved domain crucial for its enzymatic activity. The primary function of LRAT is to catalyze the esterification of retinol to produce retinyl esters, a process vital for the storage and transport of vitamin A within the body. LRAT′s plays a role in the visual cycle as it contributes to the regeneration of visual pigments in the retina. The expression of LRAT is regulated by dietary vitamin A levels, and it is found in various tissues, including the liver, lungs, testis, and the eye. LRAT is primarily localized to the endoplasmic reticulum. Mutations in the LRAT gene have been linked to a form of retinitis pigmentosa, an inherited retinal disease associated with progressive vision loss.
物理的形状
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
再構成
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABN2709-25UG:
MABN2709-100UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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