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Key Documents

安全性情報

MABN2636

Sigma-Aldrich

Anti-Arrestin-C Antibody, clone 7G6

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

7G6, monoclonal

分子量

calculated mol wt 42.67 kDa

精製方法

using protein G

化学種の反応性

human, monkey, bovine

包装

antibody small pack of 100 μg

テクニック

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

アイソタイプ

IgG1κ

エピトープ配列

N-terminal

UniProtアクセッション番号

輸送温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

詳細

Arrestin-C (UniProt: A0A2K5U9A7; also known as Cone arrestin, Retinal cone arrestin-3) is encoded by the ARR3 gene (Gene ID: 102134564) in Monkey. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C is predominantly found in inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Clone 7G6 is a cone-specific marker that recognizes all cones in the adult primate retina. In fetal retina, although long and middle wavelength-sensitive cones recognized by clone 7G6, a subset of short wavelength-sensitive cones are shown to be delayed in their reactivity to this clone. (Ref.: Zhang, H., et al. (2003). Invest. Opthalmol. Vis. Sci. 44(7); 2858-2867; Wikler, KC., et al. (1997). J. Comp. Neurol. 377(4); 500-508).

特異性

Clone 7G6 is a mouse monoclonal antibody that detects cone arrestin (Arrestin-C). It targets an epitope within the N-terminal region.

免疫原

Crude retinal extract from Monkey (Macaca fascicularis).

アプリケーション

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Tested Applications

Western Blotting Analysis: A representative lot detected Arrestin-C in Western Blotting applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).

Immunohistochemistry Applications: A representative lot detected Arrestin-C in Immunohistochemistry applications (Wikler, K.C., et al. (1997). J Comp Neurol.;377(4):500-8; John, S.K., et al. (2000). Mol Vis.;6:204-15; Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67; O′Brien, J.J., et al. (2012). J Neurosci.;32(13):4675-87).

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Arrestin-C in frozen monkey retina tissues (Courtesy of Prof. Peter MacLeish, Ph.D., photo by Talib Saafir, Ph.D., Morehouse School of Medicine, Atlanta, GA, USA).

Immunofluorescence Analysis: A representative lot detected Arrestin-C in Immunofluorescence applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Arrestin-C, clone 7G6, Cat. No. MABN2636, is a mouse monoclonal antibody that detects Arrestin-C and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABN2636-100UG:
MABN2636-25UG:


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