コンテンツへスキップ
Merck
すべての画像(2)

主要文書

安全性情報

MABN1534

Sigma-Aldrich

Anti-TREM-2 Antibody, clone 10H4.2

clone 10H4.2, from mouse

別名:

Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2

ログイン組織・契約価格を表示する


About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.43

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

10H4.2, monoclonal

交差性

human

テクニック

immunohistochemistry: suitable (paraffin)
western blot: suitable

アイソタイプ

IgG1κ

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... TREM2(54209)

詳細

Triggering receptor expressed on myeloid cells 2 (UniProt Q9NZC2; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the TREM2 gene (Gene ID 54209) in human. TREM2 is an Ig-like V-type receptor expressed by populations of myeloid cells in the central nervous system and periphery. TREM2 is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. Apolipoprotein E (apoE) is reported to function as a high-affinity TREM2 ligand. ApoE binding enhances the phagocytosis of apoptotic N2a cells by primary microglia cells, and greatly reduced ApoE binding is seen with AD-associated TREM2 R47H mutatant. TREM2 is produced with a signal peptide sequence (a.a. 1-18), the removal of which yields the mature single-transmembrane (a.a. 175-195) protein with a large extracellular region (a.a. 19-174) that contains the V-type Ig-like domain (a.a. 29-112) and a cytoplasmic tail (a.a. 196-230). The short cytoplasmic tail contains no signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction. TREM2 gene mutations and polymorphisms are linked to several neurological disorders, including Alzheimer′s disease (AD), frontotemporal dementia (FTD), Parkinson′s disease (PD), and Nasu-Hakola disease (a.k.a. PLOSL or polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy).

特異性

Clone 10H4.2 recognizes a cytoplasmic domain epitope that is not present in the spliced isoforms 2&3 reported by UniProt (Q9NZC2).

免疫原

Epitope: Cytoplasmic domain.
KLH-conjugated linear peptide corresponding to a cytoplasmic domain sequence of human TREM-2.

アプリケーション

Research Category
ニューロサイエンス
Research Sub Category
神経炎症及び疼痛
Anti-TREM-2 Antibody, clone 10H4.2 is an antibody against TREM-2 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TREM-2 in human bone marrow, spleen and cerebellum tissue sections.

品質

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.

ターゲットの説明

~30 kDa oberved. Target band size appears larger than the calculated moleuclar weights of 25.45 kDa (pro-form) and 23.45 kDa (mature form) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABN1534:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)