MABF3095

Anti-Complement C5 Antibody, clone SIM 225-3.2.1

• 別名: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: SIM 225-3.2.1, monoclonal
• 分子量: observed mol wt ~188 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: rat, guinea pig, porcine, goat, feline, canine, rabbit
• 包装: antibody small pack of 100
• テクニック: ELISA: suitable; flow cytometry: suitable; western blot: suitable
• アイソタイプ: IgG1
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_001304092
• UniProtアクセッション番号: P01031
• 保管温度: 2-8°C
• 遺伝子情報: human ... C5(727)

詳細

特異性

Clone SIM 225-3.2.1 is a mouse monoclonal antibody that detects Complement 5.

免疫原

Complement C5 from human serum.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with Human Complement C5 glycoprotein (non-reduced).

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected non-reduced Human Complement C5.

Tested Applications

Western Blotting Analysis: A representative lot detected Complement C5 in Western Blotting applications (Monedero, M., et al. (2019). Comp Immunol Microbiol Infect Dis. 62:7-12).

Flow Cytometry Analysis: A representative lot detected Complement C5 in Flow Cytometry applications (Monedero, M., et al. (2019). Comp Immunol Microbiol Infect Dis. 62:7-12).

ELISA Analysis: A representative lot detected Complement C5 in applications (Monedero, M., et al. (2019). Comp Immunol Microbiol Infect Dis. 62:7-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Complement C5 (UniProt: P01031; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4) is encoded by the C5 (also known as CPAMD4) gene (Gene ID: 727) in human. The complement system provides innate defense against microbial infection and is a complement to antibody-mediated immunity. It consists of about thirty-five interacting plasma and membrane associated proteins. Activation of the complement system is achieved through three major independent, but overlapping, pathways: the classical pathway, which is activated by certain isotypes of antibodies bound to antigens (immune complexes); the alternative pathway, which is activated on microbial cell surfaces in the absence of antibody; and the lectin pathway, which is activated by a plasma lectin (mannose binding lectin) that binds to mannose residues on microbes. The human C5 complement component is a glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that can further cleave into four chains: Complement C5 beta chain (aa 19-673); Complement C5 alpha chain (aa 678-1676); C5a anaphylatoxin (aa 678-751); and Complement C5 alpha′ chain (aa752-1676). The C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b. C5a serves as an inflammatory mediator that can activate a variety of cells through G-protein-linked receptors. The C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC) that is responsible for the membrane lytic events associated with complement activation. Mutations in C5 gene are known to cause complement component 5 deficiency (C5D), a rare defect of the complement classical pathway that leads to susceptibility to severe recurrent infections. (Ref.: Monedero, M., et al. (2019). Comp. Immunol. Microbiol. Infect. Dis. 62; 7-12; Horiuchi, T., and Tsukamoto, H. (2016). Inflamm. Regen. 36; Article 11).

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommended storage: +2°C to +8°C

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable