MABF288
Anti-Roquin-1/2, clone 3F12 Antibody
clone 3F12, from rat
別名:
Roquin-1, Roquin, RING finger and C3H zinc finger protein 1, RING finger and CCCH-type zinc finger domain-containing protein 1, RING finger protein 198
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すべての画像(1)
About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
rat
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
3F12, monoclonal
化学種の反応性
human, mouse
テクニック
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RC3H1(149041)
詳細
Roquin-1 shares 46% identity with Roquin-2 and both serve the same function and belong to the Zinc finger family of RNA binding proteins. Roquin-1/2 are cytoplasmic RNA suppression proteins that specifically bind selective RNAs and promote the deadenylation of mature mRNAs and their degradation. Roquin-1/2s targets include HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more critical RNAs. In particular, because of their impact on ICSO and gamma interferon and T helper cell activation, Roquin-1/2 are being examined as mediators of the systemic inflammation response and controllers of adaptive and innate immune responses in autoimmune disease. Roquin 1/2 are widely expressed and localized in the cytoplasm and/or P bodies and cytosolic stress granules when under cellular stress.
特異性
This antibody specifically detects both Roquin-1 & Roquin-2 (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
免疫原
Recombinant protein corresponding to human Roquin-1/2.
アプリケーション
This Anti-Roquin-1/2, clone 3F12 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Roquin-1/2.
Western Blotting Analysis: A representative lot detected Roquin-1/2 in both Roquin-1 & Roquin-2 in normal MEFs, only Roquin-2 and Roquin-1 deficient MEFs, and neither protein in Roquin-1/2 deficient MEFs. This antibody also detected Roquin-1 and Roquin-2 in Mouse Spleen, Lymph Node, Thymus, Brain & Lung tissue, CD4+ T cells and Thymocyte lysates (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Roquin-1/2 in MEF cell lysate (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Roquin-1/2 in MEF cell lysate (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
ターゲットの説明
125 kDa calculated
物理的形状
Format: Purified
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF288:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote TH17 differentiation.
Jeltsch, KM; Hu, D; Brenner, S; Zoller, J; Heinz, GA; Nagel, D; Vogel, KU; Rehage et al.
Nature Immunology null
Johannes Braun et al.
Nucleic acids research, 46(22), 12109-12125 (2018-10-09)
Post-transcriptional gene regulation controls the amount of protein produced from a specific mRNA by altering both its decay and translation rates. Such regulation is primarily achieved by the interaction of trans-acting factors with cis-regulatory elements in the untranslated regions (UTRs)
Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.
Vogel, KU; Edelmann, SL; Jeltsch, KM; Bertossi, A; Heger, K; Heinz, GA; Zoller, J; Warth et al.
Immunity null
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