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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
LP1, monoclonal
分子量
calculated mol wt 54.6 kDa
observed mol wt ~37 kDa
精製方法
using protein G
交差性
herpes simplex virus 1
包装
antibody small pack of 100 μL
テクニック
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1
エピトープ配列
Unknown
輸送温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... vp16> VP16(1487335)
詳細
Alpha trans-inducing protein (UniProt: Q69113; also known as Alpha-TIF) is encoded by the VP16 (also known as UL48) gene (Gene ID: 1487335) in human Herpes Simplex virus 2. Herpes simplex virus (HSV) is a large enveloped DNA virus that replicates in the nuclei of mammalian cells. Its virion contains two regulatory proteins that are reported to modulate gene expression in infected cells: the transcriptional activator VP16 and the virion host shutoff protein vhs. VP16, an abundant structural protein, is shown to be essential for virion assembly and it stimulates transcription of the HSV immediate-early (IE) genes. Its transcriptional activation domain is localized in the C-terminal region. It is targeted to the TAATGARATTC consensus sequence found in IE promoters through interactions with the host factors Oct-1 and HCF. It is delivered by the infecting virion and acts during the earliest stages of infection to stimulate transcription of the viral IE genes, thereby facilitating the onset of the lytic program of viral gene expression. It is also reported to suppress vhs host protein synthesis and induce accelerated turnover of cellular and viral mRNAs. Clone LP1 is directed against VP16 in both HSV-1 and HSV-2. (Ref.: Smibert, CA., et al. (1994). J. Virol. 68(4); 2339-2346; McLean, C., et al. (1982). J. Gen. Virol. 63(2); 297-305).
特異性
Clone LP1 is a mouse monoclonal antibody that detects VP16 protein of Herpes simplex virus 2 (HSV-2) and displays cross reactivity with HSV-1.
免疫原
Herpes simplex virus -2; strain 25766.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in lysate from HSV-1 infected Vero cells.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected VP16 protein in lysate from HSV-1 infected Vero cells.
Tested Applications
Immunoprecipitation Analysis): A representative lot immunoprecipitated HSV-2 VP16 in Immunoprecipitation applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Western Blotting Analysis: A representative lot detected HSV-2 VP16 in Western Blotting applications (Crameri, M., et al. (2018). Nat Commun.; 9(1):1980; McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Immunofluorescence Analysis: A representative lot detected HSV-2 VP16 in Immunofluorescence applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HSV-1 infected Vero cells.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected VP16 protein in lysate from HSV-1 infected Vero cells.
Tested Applications
Immunoprecipitation Analysis): A representative lot immunoprecipitated HSV-2 VP16 in Immunoprecipitation applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Western Blotting Analysis: A representative lot detected HSV-2 VP16 in Western Blotting applications (Crameri, M., et al. (2018). Nat Commun.; 9(1):1980; McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Immunofluorescence Analysis: A representative lot detected HSV-2 VP16 in Immunofluorescence applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HSV-2 VP16, clone LP1, Cat. No. MABF2225, is a mouse monoclonal antibody that detects Alpha trans-inducing protein (VP16) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF2225-100UL:
MABF2225-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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