MABF2033M

Anti-CD31/PECAM-1.3 Antibody, clone MBC 78.3

clone MBC 78.3, from mouse

• 別名: Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA′, PECA1, CD31
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: MBC 78.3, monoclonal
• 化学種の反応性: human, canine
• 包装: antibody small pack of 25 μg
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000433
• UniProtアクセッション番号: P16284
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PECAM1(5175)

詳細

詳細

Platelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA′, PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting ′detachment′ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.

特異性

Clone MBC 78.3 detects CD31/PECAM-1 in human and canine cells. It targets an epitope within the IgG homology domain 1.

免疫原

Epitope: extracellular domain

PECAM-1 purified from human platelets.

アプリケーション

Research Category
炎症及び免疫

Anti-CD31/PECAM-1.3, clone MBC 78.3 , Cat. No. MABF2033 is a mouse monoclonal antibody that detects PECAM1 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.

Flow Cytometry Analysis: A representative lot detected CD31/PECAM-1.3 in Flow Cytometry applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8).

Flow Cytometry Analysis: 1 µg from a representative lot detected CD31/PECAM-1.3 in THP-1 cells.

Western Blotting Analysis: A representative lot detected CD31/PECAM-1.3 in Western Blotting applications (Zhao, T., et. al. (2001). J Cell Biol. 152(1):65-73).

Agonist or Inhibitor Analysis: A representative performed agonist or Inhibitor function (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8; Reedguist, K.A., et. al. (2000). J Cell Biol. 148(6):1151-8).

Immunoprecipitation Analysis: A representative lot detected CD31/PECAM-1.3 in Immunoprecipitation applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).

Immunocytochemistry Analysis: A representative lot detected CD31/PECAM-1.3 in Immunocytochemistry applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27; Zhao, T., et. al. (2001). J Cell Biol. 152(1):65-73).

品質

Evaluated by Immunocytochemistry in un-permeablilized THP-1 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31/PECAM-1.3 in un-permeablilized THP-1 cells.

ターゲットの説明

82.54 kDa calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable