MABF1988

Anti-CMTM6 Antibody, clone RCT6

• 別名: CKLF-like MARVEL transmembrane domain-containing protein 6;Chemokine-like factor superfamily member 6
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: RCT6, monoclonal
• 分子量: calculated mol wt 20.42 kDa; observed mol wt ~20 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 包装: antibody small pack of 100 μg
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_060271
• UniProtアクセッション番号: Q9NX76
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CMTM6(54918)

詳細

詳細

CKLF-like MARVEL transmembrane domain-containing protein 6 (UniProt: Q9NX76; also known as Chemokine-like factor superfamily member 6, CMTM6) is encoded by the CMTM6 gene (Gene ID: 54918) in human. CMTM6 is a ubiquitously expressed, multi-pass membrane protein of the family of MARVEL domain-containing proteins with 3 cytoplasmic domains, 4 transmembrane domains, and 2 extracellular domains. Its MARVEL domain is localized in amino acids 33-160. It serves as a master regulator of recycling and plasma membrane expression of PD-L1. It binds to PD-L1 and maintains its cell surface expression. Although it is not required for PD-L1 maturation, but it co-localizes with PD-L1 at the plasma membrane and in recycling endosomes, where it prevents PD-L1 from being targeted for lysosome-mediated degradation by preventing its STUB-1 mediated ubiquitination. It also stabilizes PD-L1 expression on antigen presenting cells and potentiates inhibitory signaling by PD-1 (CD279), ultimately triggering T-cell anergy. It enhances the ability of PD-L1-expressing tumor cells to inhibit T cell activity. (Ref.: Burr, ML., et al. (2017). Nature. 549(7670); 101-105; Mezzadra, R., et al. (2017). Nature. 549(7670); 106-110).

特異性

Clone RCT6 is a mouse monoclonal antibody that detects human CMTM6. It targets an epitope within the C-terminal region.

免疫原

A linear peptide corresponding to a sequence from the C-terminal region of human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6).

アプリケーション

Quality Control Testing

Evaluated by Western blotting in lysates from 8505c cells overexpressing CMTM6.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CMTM6 in lysates from 8505C cells overexpressing CMTM6, but not in knockout or untransfected cells.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CMTM6 in Immunohistochemistry applications (Mezzadra, R., et al. (2017). Nature. 549(7670):106-110).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CMTM6, clone RCT6, Cat. No. MABF1988, is a mouse monoclonal antibody that detects CMTM6 and is tested for use in Immunohistochemistry and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable