MABE867
Anti-Mad1 Antibody, clone BB3-8
clone BB3-8, from mouse
別名:
Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181
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すべての画像(1)
About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
BB3-8, monoclonal
交差性
human
テクニック
immunofluorescence: suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MAD1L1(8379)
詳細
Mitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20.
免疫原
His-tagged recombinant protein corresponding to human Mad1.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
細胞周期、DNA複製及び修復
細胞周期、DNA複製及び修復
Anti-Mad1 Antibody, clone BB3-8 is a highly specific mouse monoclonal antibody, that targets MAD1 & has been tested in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
品質
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
ターゲットの説明
~83 kDa observed
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE867:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Emanuela Screpanti et al.
Current biology : CB, 21(5), 391-398 (2011-03-01)
Kinetochores are proteinaceous scaffolds implicated in the formation of load-bearing attachments of chromosomes to microtubules during mitosis. Kinetochores contain distinct chromatin- and microtubule-binding interfaces, generally defined as the inner and outer kinetochore, respectively (reviewed in). The constitutive centromere-associated network (CCAN)
Shawn Yost et al.
Nature genetics, 49(7), 1148-1151 (2017-05-30)
Through exome sequencing, we identified six individuals with biallelic loss-of-function mutations in TRIP13. All six developed Wilms tumor. Constitutional mosaic aneuploidies, microcephaly, developmental delay and seizures, which are features of mosaic variegated aneuploidy (MVA) syndrome, were more variably present. Through
Stefano Santaguida et al.
The EMBO journal, 30(8), 1508-1519 (2011-03-17)
Fidelity of chromosome segregation is ensured by a tension-dependent error correction system that prevents stabilization of incorrect chromosome-microtubule attachments. Unattached or incorrectly attached chromosomes also activate the spindle assembly checkpoint, thus delaying mitotic exit until all chromosomes are bioriented. The
Adrian T Saurin et al.
Methods in molecular biology (Clifton, N.J.), 1413, 333-347 (2016-05-20)
Mitotic kinetochores are signaling network hubs that regulate chromosome movements, attachment error-correction, and the spindle assembly checkpoint. Key switches in these networks are kinases and phosphatases that enable rapid responses to changing conditions. Describing the mechanisms and dynamics of their
Lindsey A Allan et al.
The EMBO journal, 39(12), e103180-e103180 (2020-03-24)
Cyclin B:CDK1 is the master kinase regulator of mitosis. We show here that, in addition to its kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help preserve genome stability. Cyclin B1 localises to an expanded region
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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