おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
H-20, monoclonal
化学種の反応性（ホモロジーによる予測）
all
テクニック
dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1κ
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
詳細
The nucleotide structure named the 2,2,7-trimethylguanosine(m3G)-containing cap structure of small nuclear ribonucleoprotein particles, or U snRNPs is an essential part of mRNA processing. Each snRNP particle consists of one (U1, U2 and U5) or two (U4/U6) snRNA molecules, a common set of eight core proteins (B, B9, D1, D2, D3, E, F and G, also denoted Sm proteins) that are bound to each of the 2,2,7- trimethylguanosine (m3G) cap-containing snRNAs U1, U2, U4 and U5, and several proteins associated specifically with the individual U snRNPs. Except for U6 snRNP, which does not leave the nucleus, the synthesis of these U snRNPs requires the bidirectional transport of the snRNA across the nuclear envelope. The snRNAs U1, U2, U4 and U5 are synthesized in the nucleus with a 7-monomethylguanosine (m7G) cap structure whereas the Sm proteins are stored in the cytoplasm and do not migrate into the nucleus in the absence of bound U snRNA. Instead, newly transcribed U snRNAs are transiently exported into the cytoplasm where the Sm proteins bind the snRNA’s Sm site, to form a ribonucleoprotein complex referred to as the Sm core. Stable association of all Sm proteins is essential for the hypermethylation of the m7G-cap to the m3G-cap structure. After this event and processing of the snRNAs, the mature snRNP particles are transported back to the nucleus in a receptor-and energy-dependent manner and the complete particle is formed. Monoclonal H-20 recognizes both m3G cap containing snRNPs as well as m7G capped structures and it should have a wide application, not only for studying the molecular biology and immunology of the U snRNPs from diverse organisms, but also for the characterization and isolation of m7G-capped transcripts.
免疫原
Human m3G-cap and m7G-cap
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
エピジェネティクス
Anti-m3G-cap, m7G-cap Antibody, clone H-20 is a mouse monoclonal antibody, validated for use in Dot Blot, IP, western blotting & ICC.
Immunoprecipitation Analysis: A representative lot immunoprecipitated m3G-capped and m7G-capped Xenopus EF-1a, which was spiked into 4 µg of HeLa total RNA.
Western Blotting Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped snRNAs from HeLa nuclear extracts (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated m3G-cap from prefractionated HeLa S100 extracts (Huber, J., et al. (1998). EMBO J. 17(14):4114-4126.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped U1 snRNPs from digitonin permeabilized HeLa cells (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).
Western Blotting Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped snRNAs from HeLa nuclear extracts (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated m3G-cap from prefractionated HeLa S100 extracts (Huber, J., et al. (1998). EMBO J. 17(14):4114-4126.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped U1 snRNPs from digitonin permeabilized HeLa cells (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).
品質
Evaluated by Dot Blot in in vitro transcribed RNA without and with m7G-cap.
Dot Blot Analysis: 1 µg/mL of this antibody detected m7G-cap in in vitro transcribed RNA containing m7G-cap.
Dot Blot Analysis: 1 µg/mL of this antibody detected m7G-cap in in vitro transcribed RNA containing m7G-cap.
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE419:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.
Bochnig, P, et al.
European Journal of Biochemistry, 168, 461-467 (1987)
Yasutoshi Akiyama et al.
RNA biology, 17(8), 1116-1124 (2020-03-03)
Recent transcriptome-wide studies have identified a diverse pool of transfer RNA (tRNA)-derived RNAs or tRNA-derived fragments (tRFs). Some of these RNAs have been demonstrated to be functional and involved in multiple biological processes ranging from the regulation of gene expression
Abhijit S Deshmukh et al.
Scientific reports, 6, 35288-35288 (2016-10-21)
Cyclin-dependent kinase 7 in conjunction with CyclinH and Mat1 activates cell cycle CDKs and is a part of the general transcription factor TFIIH. Role of Cdk7 is well characterized in model eukaryotes however its relevance in protozoan parasites has not
J Huber et al.
The EMBO journal, 17(14), 4114-4126 (1998-07-22)
The nuclear import of the spliceosomal snRNPs U1, U2, U4 and U5, is dependent on the presence of a complex nuclear localization signal (NLS). The latter is composed of the 5'-2,2,7-terminal trimethylguanosine (m3G) cap structure of the U snRNA and
Francesco Neri et al.
Nature, 543(7643), 72-77 (2017-02-23)
In mammals, DNA methylation occurs mainly at CpG dinucleotides. Methylation of the promoter suppresses gene expression, but the functional role of gene-body DNA methylation in highly expressed genes has yet to be clarified. Here we show that, in mouse embryonic
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）