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詳細
HU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
特異性
This reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F.
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
免疫グロブリン及び免疫
免疫グロブリン及び免疫
Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.
Flow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer.
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects/Fluorescence Intensity
Heterozygous HLA-B27+/Strong
Homozygous HLA-B7+/Weak
Heterozygous HLA-B7+/Weak - negative
Non B27, Non B7/Negative
This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.
SUGGESTED USAGE:
Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer.
Use Control IgG2a-FITC (MABC002F) as an isotype control.
Please note that this reagent should be used in a single-colour single-step assay only.
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects/Fluorescence Intensity
Heterozygous HLA-B27+/Strong
Homozygous HLA-B7+/Weak
Heterozygous HLA-B7+/Weak - negative
Non B27, Non B7/Negative
This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.
SUGGESTED USAGE:
Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer.
Use Control IgG2a-FITC (MABC002F) as an isotype control.
Please note that this reagent should be used in a single-colour single-step assay only.
品質
Evaluated by Western Blot on Jurkat lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates.
ターゲットの説明
19 kDa
物理的形状
The reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry.
保管および安定性
Stable for 6 months at 2-8ºC in the dark from date of receipt.
アナリシスノート
Control
Control IgG2a-FITC (MABC002F) as an isotype control
Control IgG2a-FITC (MABC002F) as an isotype control
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB4212F:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
Trapani, J A, et al.
Human immunology, 7, 205-216 (1983)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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