MAB4145

Anti-BCRP Antibody, clone BXP-34

culture supernatant, clone BXP-34, Chemicon^®

• 別名: Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: culture supernatant
• 抗体製品タイプ: primary antibodies
• クローン: BXP-34, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable
• 入力: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• アイソタイプ: IgG1
• 適合性: not suitable for Western blot; not suitable for immunohistochemistry (Paraffin)
• NCBIアクセッション番号: NM_004827.2
• UniProtアクセッション番号: Q9UNQ0
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ABCG2(9429)

詳細

詳細

The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates.

特異性

This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.

免疫原

The Mitoxanthrone resistant, BCRP-overexpressing cell line MCF7-MR.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
造血幹細胞

Detect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.

Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections

Not suitable for paraffin-embedded tissues or for Western blotting

Optimal working dilutions must be determined by end user.

ターゲットの説明

70 kDa

物理的形状

Liquid containing 0.7% BSA and 0.1% sodium azide.

保管および安定性

Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.

アナリシスノート

Control
MCF-7 cells

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1