おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
N5/22, monoclonal
化学種の反応性
rabbit, bovine, human, pig
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CALD1(800)
詳細
Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils. It
is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a
Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon)
and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle
while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of
binding two calmodulin molecules, one at either end of the protein. Additionally,
Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon
is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits
the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an
important function in motile processes such as secretion and organelle movement.
is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a
Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon)
and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle
while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of
binding two calmodulin molecules, one at either end of the protein. Additionally,
Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon
is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits
the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an
important function in motile processes such as secretion and organelle movement.
特異性
Caldesmon, smooth muscle. By Western blot the antibody recognizes a protein of 150-kDa.
免疫原
Crude smooth muscle extract from normal human adult uterus.
Epitope: smooth muscle
アプリケーション
Research Category
代謝
代謝
Research Sub Category
筋生理学
筋生理学
Detect Caldesmon using this Anti-Caldesmon Antibody, smooth muscle, clone N5/22 validated for use in IP, WB, IC, IH(P).
Western blot. Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 7% and/or 4-20% (or similar) gradient gel.
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.
Immunocytochemistry
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.
Immunocytochemistry
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.
Optimal working dilutions must be determined by the end user.
関連事項
Replaces: 04-590
物理的形状
Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
保管および安定性
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt
アナリシスノート
Control
POSITIVE CONTROL:
Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).
POSITIVE CONTROL:
Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB3576:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jonathan Paul et al.
PloS one, 6(6), e21542-e21542 (2011-07-09)
Human myometrium develops phasic contractions during labor. Phosphorylation of caldesmon (h-CaD) and extracellular signal-regulated kinase 1/2 (ERK 1/2) has been implicated in development of these contractions, however the phospho-regulation of these proteins is yet to be examined during periods of
Alvaro Yogi et al.
Biomedicines, 9(7) (2021-08-07)
Synthetic grafts have been developed for vascular bypass surgery, however, the risks of thrombosis and neointimal hyperplasia still limit their use. Tissue engineering with the use of adipose-derived stem cells (ASCs) has shown promise in addressing these limitations. Here we
M G Frid et al.
Developmental biology, 153(2), 185-193 (1992-10-01)
Expression of the regulatory contractile proteins, heavy caldesmon (h-caldesmon) and calponin was studied in human aortic smooth muscle cells (SMCs) during development and compared with the expression of alpha-SM-actin and smooth muscle-myosin heavy chain (SM-MHCs). For this study, novel monoclonal
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）