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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
クローン
R4A, monoclonal
化学種の反応性
pig, chicken, monkey, canine, feline, human, hamster
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SMTN(6525)
詳細
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
特異性
MAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.
免疫原
Cytoskeletal extract of chicken gizzard.
アプリケーション
Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).
Western blot
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.
物理的形状
Format: Purified
Liquid in buffer (PBS) with 0.1% sodium azide.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB3242:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Yan Zhang et al.
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, 19(6), e233-e240 (2010-01-23)
We have recently shown that native murine ventricular fibroblasts express both connexin43 (Cx43) and Cx45, and that the level of Cx43 expression influences intercellular coupling and cell proliferation. Relatively little is known, however, about how myocardial infarction (MI) influences expression
Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype.
Hector F Simosa, Grace Wang, XinXin Sui, Timothy Peterson, Vinod Narra, Dario C Altieri et al.
Journal of Vascular Surgery null
Eric van der Veer et al.
Circulation research, 97(1), 25-34 (2005-06-11)
Conversion of vascular smooth muscle cells (SMCs) from a proliferative state to a nonproliferative, contractile state confers vasomotor function to developing and remodeling blood vessels. Using a maturation-competent human SMC line, we determined that this shift in phenotype was accompanied
Sachiko Matsuzaki et al.
Human reproduction (Oxford, England), 31(3), 541-553 (2016-01-15)
Can deep infiltrating endometriotic stromal cells (DES) sense changes in extracellular matrix (ECM) stiffness and respond to them? Soft matrices inhibit cell proliferation and inactivate the fibrotic phenotype of DES in vitro. Deep infiltrating endometriosis (DIE) is characterized histologically by
Philippe Gomis et al.
Journal of neurosurgery, 102(4), 715-720 (2005-05-06)
The aim of this project was to study the perturbations of four smooth-muscle proteins and an extracellular protein, type I collagen, after subarachnoid hemorrhage (SAH) and to examine the possible preventive effects of dexamethasone. Using a one-hemorrhage rabbit model, the
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