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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
4D1, monoclonal
化学種の反応性
human, rat
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... GPC1(2817)
rat ... Gpc1(58920)
詳細
Heparan sulfate proteoglycans (HSPGs) can be divided into general categories of
cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008).
Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008).
cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008).
Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008).
特異性
MAB2600 recognizes glypican-1.
免疫原
Epitope: Full Length
Full length recombinant protein.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
ECMタンパク質
ECMタンパク質
Anti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB.
Immunohistochemistry:
A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells.
A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells.
品質
Routinely evaluated by Western blot on human PC-12 cell lysate using 0.5 µg/mL of Anti-Glypican-1 at 1 mg/mL.
ターゲットの説明
Recognizes Glypican-1 at ~ 59 kDa
物理的形状
Protein G Purified
Format: Purified
Purified in 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% NaN3.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
PC-12 cell lysate.
PC-12 cell lysate.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB2600:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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