MAB2041-I

Anti-Laminin β1 Antibody, clone 3E5

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 3E5, monoclonal
• 分子量: calculated mol wt 198.04 kDa; observed mol wt ~200 kDa
• 精製方法: using protein G
• 化学種の反応性: rat, human
• 包装: antibody small pack of 100 μL
• テクニック: ELISA: suitable; electron microscopy: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_002282
• UniProtアクセッション番号: P07942
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... lamb1> LAMB1(3912)

詳細

詳細

Laminin subunit b1 (UniProt: P07942; also known as Laminin B1 chain, Laminin-1 subunit beta) is encoded by the LAMB1 gene (Gene ID: 3912) in human. Laminins are the major non-collagenous constituent of basement membranes that participate in wide variety of biological processes, including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. They are a family of heterotrimeric extracellular glycoproteins consisting of α, β, and γ chains that are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five α, three β, and three γ subunits are described that assemble into at least 15 different laminin molecules. Different α, β, and γ chain isomers combine to give rise to different heterotrimeric laminin isoforms, which are designated by Arabic numerals in the order of their discovery. Laminin- β1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. It is expressed in most tissues that produce basement membranes and is one of the 3 chains constituting laminin 1. It is shown to be involved in the organization of the laminar architecture of cerebral cortex and is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the end feet of radial glial cells and as a physical barrier to migrating neurons. (Ref.: Radmanesh, F., et al. (2013). Am. J. Hum. Genet. 92(3); 468-474; Virtanen, I., et al. (1995). J. Histochem. Cytochem. 43(6); 621-628)).

This mouse monoclonal Anti-Laminin β1, clone 3E5, Cat. No. MAB2041-I is tested for use in Western Blotting and ELISA, Inhibition, Electron Microscopy and Western Blotting, for the detection of Laminin subunit β1.

特異性

Clone 3E5 is a mouse monoclonal antibody that detects Laminin subunit β1. It targets an epitope near the end of long arm.

免疫原

Laminin fragments from human placenta.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in Human placenta tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Laminin β1 in Human placenta tissue lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected Laminin β1 in Western Blotting applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

Electron Microscopy: A representative lot detected Laminin β1 in Electron Microscopy applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

Inhibition: A representative lot inhibited the neurite-promoting activity of laminin. (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

ELISA Analysis: A representative lot detected Laminin β1 in ELISA applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

物理的形状

Purified mouse monoclonal antibody IgG in PBS without azide.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable