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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
25E11, monoclonal
化学種の反応性
human
メーカー／製品名
Chemicon®
テクニック
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
アイソタイプ
IgM
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ITGB3(3690)
特異性
Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. The apparent molecular weight of the GPIIIa by SDS-PAGE is 105 kDa reduced and 90 kDa unreduced. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.
(original paper claimed IgG2ak)
(original paper claimed IgG2ak)
免疫原
Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
インテグリン
インテグリン
Immunoprecipitation
Immunocytochemistry (acetone fixation required)
Flow cytometry
Inhibits platelet aggregation, 10 μg/mL
Optimal working dilutions must be determined by end user.
Immunocytochemistry (acetone fixation required)
Flow cytometry
Inhibits platelet aggregation, 10 μg/mL
Optimal working dilutions must be determined by end user.
This Anti-Integrin β3 Antibody, clone 25E11 is validated for use in FC, IP, IC, FUNC for the detection of Integrin β3.
関連事項
Replaces: 04-1060
物理的形状
Format: Purified
In buffer of 0.02M phosphate, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.
保管および安定性
Maintain between 2 and 8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1957:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
[Laminin alpha3 chain-derived peptide promotes keratinocyte migration and wound closure: clustering of syndecan-4 and integrin beta1].
Atsushi Utani
Seikagaku. The Journal of Japanese Biochemical Society null
Francisco M Vega et al.
Open biology, 2(5), 120076-120076 (2012-06-23)
The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension
S S Akimov et al.
The Journal of cell biology, 148(4), 825-838 (2000-02-23)
The protein cross-linking enzyme tissue transglutaminase binds in vitro with high affinity to fibronectin via its 42-kD gelatin-binding domain. Here we report that cell surface transglutaminase mediates adhesion and spreading of cells on the 42-kD fibronectin fragment, which lacks integrin-binding
Identification of CD47/integrin-associated protein and alpha(v)beta3 as two receptors for the alpha3(IV) chain of type IV collagen on tumor cells.
T A Shahan, Z Ziaie, S Pasco, A Fawzi, G Bellon, J C Monboisse, N A Kefalides
Cancer Research null
Expression patterns of integrins on quiescent and invasive smooth muscle cells and impact on cell locomotion.
Rudiger Blindt, Nicole Krott, Peter Hanrath, Jurgen vom Dahl, Guillaume van Eys et al.
Journal of Molecular and Cellular Cardiology null
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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