MAB1683

Anti-Ankyrin Antibody, clone Ank016

clone Ank016, Chemicon^®, from mouse

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: Ank016, monoclonal
• application: ELISA; FACS; ICC; IP; WB
• 化学種の反応性: rat, human, rabbit, pig, mouse, bovine, canine
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 4

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: Ank016, monoclonal
• 化学種の反応性: rat, human, rabbit, pig, mouse, bovine, canine
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2a
• NCBIアクセッション番号: NM_000037.3; NM_020475.2; NM_020476.2; NM_020477.2; NM_020478.3; NM_020479.2; NM_020480.3; NM_020481.2
• UniProtアクセッション番号: P16157
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ANK1(286)

詳細

特異性

Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).

免疫原

Purified human erythroid cells ankyrin

アプリケーション

Research Sub Category
細胞骨格

Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.

Research Category
Cell Structure

Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals.

ELISA: 1:1000

Immunoprecipitation: 1:100

Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.

Flow cytometry: 1:500

Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.

RIA: 1:5000

Optimal working dilutions must be determined by end user.

物理的形状

Format: Purified

Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable