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由来生物
mouse
品質水準
結合体
biotin conjugate
抗体製品の状態
culture supernatant
抗体製品タイプ
primary antibodies
クローン
B7, monoclonal
化学種の反応性
human
メーカー／製品名
Chemicon®
テクニック
dot blot: suitable
immunohistochemistry: suitable
アイソタイプ
IgG1κ
適合性
not suitable for Western blot
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CMA1(1215)
詳細
Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
特異性
Reacts with human mast cell chymase.
免疫原
Chymase purified from human skin.
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
炎症及び自己免疫疾患
炎症及び自己免疫疾患
Immunohistochemistry at 1:2,000:1:4,000.
For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.
物理的形状
Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.
保管および安定性
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
アナリシスノート
Control
POSITIVE CONTROL: lung, heart, skin, placenta.
POSITIVE CONTROL: lung, heart, skin, placenta.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1254B:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
J H McKerrow et al.
Molecular medicine (Cambridge, Mass.), 6(5), 450-460 (2000-08-22)
Proteases facilitate several steps in cancer progression. To identify proteases most suitable for drug targeting, actual enzyme activity and not messenger RNA levels or immunoassay of protein is the ideal assay readout. An automated microtiter plate assay format was modified
Highly increased numbers of leukocytes in inflamed gingiva from patients with HIV infection.
Maung Myint, Svein Steinsvoll, Zuanning N Yuan, Berit Johne, Kristen Helgeland, Karl Schenck
AIDS null
Phenotypic change and accumulation of smooth muscle cells in strictures in Crohn's disease: relevance to local angiotensin II system.
Takehisa Suekane,Yoshihiro Ikura,Kenji Watanabe,Junko Arimoto,Yoko Iwasa,Yoshimi Sugama et al.
Journal of Gastroenterology null
Fixation with Carnoy's fluid reduces the number of chymase-positive mast cells: not all chymase-positive mast cells are also positive for tryptase
KleinJan, A. et al.
Allergy, 51, 614-620 (1996)
Arnold S Kirshenbaum et al.
PloS one, 11(7), e0159177-e0159177 (2016-07-28)
Hermansky-Pudlak Syndrome type-1 (HPS-1) is an autosomal recessive disorder caused by mutations in HPS1 which result in reduced expression of the HPS-1 protein, defective lysosome-related organelle (LRO) transport and absence of platelet delta granules. Patients with HPS-1 exhibit oculocutaneous albinism
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